PMID- 10625641
OWN - NLM
STAT- MEDLINE
DCOM- 20000218
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 2
DP  - 2000 Jan 14
TI  - Nramp2 expression is associated with pH-dependent iron uptake across the apical
      membrane of human intestinal Caco-2 cells.
PG  - 1023-9
AB  - The absorption of dietary non-heme iron by intestinal enterocytes is crucial to
      the maintenance of body iron homeostasis. This process must be tightly regulated 
      since there are no distinct mechanisms for the excretion of excess iron from the 
      body. An insight into the cellular mechanisms has recently been provided by
      expression cloning of a divalent cation transporter (DCT1) from rat duodenum and 
      positional cloning of its human homologue, Nramp2. Here we demonstrate that
      Nramp2 is expressed in the apical membrane of the human intestinal epithelial
      cell line, Caco 2 TC7, and is associated with functional iron transport in these 
      cells with a substrate preference for iron over other divalent cations. Iron
      transport occurs by a proton-dependent mechanism, exhibiting a concurrent
      intracellular acidification. Taken together, these data suggest that the
      expression of the Nramp2 transporter in human enterocytes may play an important
      role in intestinal iron absorption.
FAU - Tandy, S
AU  - Tandy S
AD  - School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ,
      United Kingdom.
FAU - Williams, M
AU  - Williams M
FAU - Leggett, A
AU  - Leggett A
FAU - Lopez-Jimenez, M
AU  - Lopez-Jimenez M
FAU - Dedes, M
AU  - Dedes M
FAU - Ramesh, B
AU  - Ramesh B
FAU - Srai, S K
AU  - Srai SK
FAU - Sharp, P
AU  - Sharp P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Iron-Binding Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (natural resistance-associated macrophage protein 1)
RN  - 0 (solute carrier family 11- (proton-coupled divalent metal ion transporters),
      member 2)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Caco-2 Cells
MH  - Carrier Proteins/genetics/*metabolism
MH  - *Cation Transport Proteins
MH  - Cell Membrane/physiology
MH  - Cloning, Molecular
MH  - Duodenum/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Intestinal Mucosa/*physiology
MH  - Iron/*metabolism
MH  - *Iron-Binding Proteins
MH  - Kinetics
MH  - Membrane Proteins/genetics/*metabolism
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Tumor Cells, Cultured
EDAT- 2000/01/08 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/08 09:00
PHST- 2000/01/08 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/08 09:00 [entrez]
AID - 10.1074/jbc.275.2.1023 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 14;275(2):1023-9. doi: 10.1074/jbc.275.2.1023.