PMID- 10625627
OWN - NLM
STAT- MEDLINE
DCOM- 20000218
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 2
DP  - 2000 Jan 14
TI  - The kappaB and V(D)J recombination signal sequence binding protein KRC regulates 
      transcription of the mouse metastasis-associated gene S100A4/mts1.
PG  - 913-20
AB  - A kappaB-like sequence, Sb, is integral to the composite enhancer located in the 
      first intron of the metastasis-associated gene, S100A4/mts1. Oligonucleotides
      containing this sequence form three specific complexes with nuclear proteins
      prepared from S100A4/mts1-expressing CSML100 adenocarcinoma cells. Protein
      studies show the Sb-interacting complexes include NF-kappaB/Rel proteins, p50.p50
      and p50.p65 dimers. Additionally, the Sb sequence was bound by an unrelated
      approximately 200-kDa protein, p200. Site-directed mutagenesis in conjunction
      with transient transfections indicate that p200, but not the NF-kappaB/Rel
      proteins, transactivates S100A4/mts1. To identify candidate genes for p200,
      double-stranded DNA probes containing multiple copies of Sb were used to screen a
      randomly primed lambdagt11 cDNA expression library made from CSML100 poly(A)(+)
      RNA. Two clones corresponding to the DNA-binding proteins KRC and Alf1 were
      identified. KRC encodes a large zinc finger protein that binds to the kappaB
      motif and to the signal sequences of V(D)J recombination. In vitro DNA binding
      assays using bacterially expressed KRC fusion proteins, demonstrate specific
      binding of KRC to the Sb sequence. In addition, introduction of KRC expression
      vectors into mammalian cells induces expression of S100A4/mts1 and reporter genes
      driven by S100A4/mts1 gene regulatory sequences. These data indicate that KRC
      positively regulates transcription of S100A4/mts1.
FAU - Hjelmsoe, I
AU  - Hjelmsoe I
AD  - Danish Cancer Society, Department of Molecular Cancer Biology, Strandboulevarden 
      49, DK-2100 Copenhagen, Denmark.
FAU - Allen, C E
AU  - Allen CE
FAU - Cohn, M A
AU  - Cohn MA
FAU - Tulchinsky, E M
AU  - Tulchinsky EM
FAU - Wu, L C
AU  - Wu LC
LA  - eng
GR  - GM48798/GM/NIGMS NIH HHS/United States
GR  - P30 CA16058/CA/NCI NIH HHS/United States
GR  - T32/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hivep3 protein, mouse)
RN  - 0 (NF-kappa B)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (S100 Calcium-Binding Protein A4)
RN  - 0 (S100 Proteins)
RN  - 0 (S100a4 protein, mouse)
RN  - 0 (S100a4 protein, rat)
RN  - 0 (Tcf12 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 142661-93-6 (TCF12 protein, human)
RN  - 142662-27-9 (S100A4 protein, human)
RN  - EC 2.7.7.- (DNA Nucleotidyltransferases)
RN  - EC 2.7.7.- (VDJ Recombinases)
SB  - IM
MH  - 3T3 Cells
MH  - 5' Untranslated Regions/genetics
MH  - Adenocarcinoma
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Binding Sites
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA Nucleotidyltransferases/metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Dimerization
MH  - Enhancer Elements, Genetic
MH  - *Gene Expression Regulation
MH  - Gene Library
MH  - Helix-Loop-Helix Motifs
MH  - Humans
MH  - Introns
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - NF-kappa B/*metabolism
MH  - Nuclear Proteins/metabolism
MH  - Rats
MH  - Recombinant Fusion Proteins/metabolism
MH  - Recombinant Proteins/metabolism
MH  - S100 Calcium-Binding Protein A4
MH  - S100 Proteins/biosynthesis/*genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - TATA Box
MH  - Transcription Factors/metabolism
MH  - *Transcription, Genetic
MH  - Tumor Cells, Cultured
MH  - VDJ Recombinases
EDAT- 2000/01/08 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/08 09:00
PHST- 2000/01/08 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/08 09:00 [entrez]
AID - 10.1074/jbc.275.2.913 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 14;275(2):913-20. doi: 10.1074/jbc.275.2.913.