PMID- 10625619
OWN - NLM
STAT- MEDLINE
DCOM- 20000218
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 2
DP  - 2000 Jan 14
TI  - Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.
PG  - 861-6
AB  - We describe the characterization of siglec-8, a novel sialic acid-binding
      immunoglobulin-like lectin that is expressed specifically by eosinophils. A
      full-length cDNA encoding siglec-8 was isolated from a human eosinophil cDNA
      library. Siglec-8 is predicted to contain three extracellular immunoglobulin-like
      domains, a transmembrane region, and a cytoplasmic tail of 47 amino acids. The
      siglec-8 gene mapped on chromosome 19q13.33-41, closely linked to genes encoding 
      CD33 (siglec-3), siglec-5, siglec-6, and siglec-7. When siglec-8 was expressed on
      COS cells or as a recombinant protein fused to the Fc region of human IgG(1), it 
      was able to mediate sialic acid-dependent binding to human erythrocytes and to
      soluble sialoglycoconjugates. Using specific monoclonal antibodies, siglec-8
      could be detected only on eosinophils and hence appears to be the first example
      of an eosinophil-specific transmembrane receptor.
FAU - Floyd, H
AU  - Floyd H
AD  - The Wellcome Trust Biocentre at Dundee, Department of Biochemistry, University of
      Dundee, Dundee DD1 5EH, Scotland, United Kingdom.
FAU - Ni, J
AU  - Ni J
FAU - Cornish, A L
AU  - Cornish AL
FAU - Zeng, Z
AU  - Zeng Z
FAU - Liu, D
AU  - Liu D
FAU - Carter, K C
AU  - Carter KC
FAU - Steel, J
AU  - Steel J
FAU - Crocker, P R
AU  - Crocker PR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD33 protein, human)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lectins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SIGLEC7 protein, human)
RN  - 0 (SIGLEC8 protein, human)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 3)
RN  - 0 (Sialic Acids)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/*chemistry/*genetics/metabolism
MH  - Antigens, Differentiation, B-Lymphocyte/*chemistry/*genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/chemistry/genetics
MH  - COS Cells
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 19
MH  - Eosinophils/*immunology
MH  - Erythrocytes/immunology
MH  - Gene Library
MH  - Genetic Linkage
MH  - Granulocytes/immunology
MH  - Humans
MH  - Immunoglobulin Fc Fragments/genetics
MH  - Immunoglobulin G/genetics
MH  - *Lectins
MH  - Lymphocytes/immunology
MH  - Molecular Sequence Data
MH  - Monocytes/immunology
MH  - Recombinant Fusion Proteins/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sialic Acid Binding Ig-like Lectin 3
MH  - Sialic Acids/blood
MH  - Transfection
EDAT- 2000/01/08 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/08 09:00
PHST- 2000/01/08 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/08 09:00 [entrez]
AID - 10.1074/jbc.275.2.861 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 14;275(2):861-6. doi: 10.1074/jbc.275.2.861.