PMID- 10623794
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20220223
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 2
DP  - 2000 Jan 15
TI  - Cutting edge: heat shock protein 60 is a putative endogenous ligand of the
      toll-like receptor-4 complex.
PG  - 558-61
AB  - Human heat shock protein 60 (hsp60) elicits a potent proinflammatory response in 
      cells of the innate immune system and therefore has been proposed as a danger
      signal of stressed or damaged cells. We report here that macrophages of C3H/HeJ
      mice, carrying a mutant Toll-like-receptor (Tlr) 4 are nonresponsive to hsp60.
      Both the induction of TNF-alpha and NO formation were found dependent on a
      functional Tlr4 whereas stimulation of macrophages by CpG DNA was Tlr4
      independent. We conclude that Tlr4 mediates hsp60 signaling. This is the first
      report of a putative endogenous ligand of the Tlr4 complex.
FAU - Ohashi, K
AU  - Ohashi K
AD  - German Diabetes Research Institute, Heinrich-Heine-University, Dusseldorf,
      Germany.
FAU - Burkart, V
AU  - Burkart V
FAU - Flohe, S
AU  - Flohe S
FAU - Kolb, H
AU  - Kolb H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Chaperonin 60)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Ligands)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Chaperonin 60/*metabolism/physiology
MH  - *Drosophila Proteins
MH  - Humans
MH  - Ligands
MH  - Macromolecular Substances
MH  - Macrophages/immunology/metabolism
MH  - Membrane Glycoproteins/*metabolism/physiology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Nitric Oxide/biosynthesis
MH  - Receptors, Cell Surface/*metabolism/physiology
MH  - Signal Transduction/immunology
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
MH  - Tumor Necrosis Factor-alpha/biosynthesis
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - ji_v164n2p558 [pii]
AID - 10.4049/jimmunol.164.2.558 [doi]
PST - ppublish
SO  - J Immunol. 2000 Jan 15;164(2):558-61. doi: 10.4049/jimmunol.164.2.558.