PMID- 10623671
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20181130
IS  - 0002-9440 (Print)
IS  - 0002-9440 (Linking)
VI  - 156
IP  - 1
DP  - 2000 Jan
TI  - Localization of human acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) in 
      macrophages and in various tissues.
PG  - 227-36
AB  - To investigate the distribution of acyl-coenzyme A:cholesterol acyltransferase-1 
      (ACAT-1) in various human tissues, we examined tissues of autopsy cases
      immunohistochemically. ACAT-1 was demonstrated in macrophages, antigen-presenting
      cells, steroid hormone-producing cells, neurons, cardiomyocytes, smooth muscle
      cells, mesothelial cells, epithelial cells of the urinary tracts, thyroid
      follicles, renal tubules, pituitary, prostatic, and bronchial glands, alveolar
      and intestinal epithelial cells, pancreatic acinar cells, and hepatocytes. These 
      findings showed that ACAT-1 is present in a variety of human tissues examined.
      The immunoreactivities are particularly prominent in the macrophages, steroid
      hormone-producing cells, followed by hepatocytes, and intestinal epithelia. In
      cultured human macrophages, immunoelectron microscopy revealed that ACAT-1 was
      located mainly in the tubular rough endoplasmic reticulum; immunoblot analysis
      showed that the ACAT-1 protein content did not change with or without cholesterol
      loading; however, on cholesterol loading, about 30 to 40% of the total
      immunoreactivity appeared in small-sized vesicles. These vesicles were also
      enriched in 78-kd glucose-regulated protein (GRP 78), a specific marker for the
      endoplasmic reticulum. Immunofluorescent microscopy demonstrated extensive
      colocalization of ACAT-1 and GRP 78 signals in both the tubular and vesicular
      endoplasmic reticulum before and after cholesterol loading. These results raise
      the possibility that foam cell formation may activate an endoplasmic reticulum
      vesiculation process, producing vesicles enriched in the ACAT-1 protein.
FAU - Sakashita, N
AU  - Sakashita N
AD  - Second Department of Pathology, Kumamoto University School of Medicine, Kumamoto,
      Japan. naomi@kaiju.medic.kumamoto-u.ac.jp
FAU - Miyazaki, A
AU  - Miyazaki A
FAU - Takeya, M
AU  - Takeya M
FAU - Horiuchi, S
AU  - Horiuchi S
FAU - Chang, C C
AU  - Chang CC
FAU - Chang, T Y
AU  - Chang TY
FAU - Takahashi, K
AU  - Takahashi K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Carrier Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.3.1.26 (Sterol O-Acyltransferase)
RN  - YCYIS6GADR (molecular chaperone GRP78)
SB  - AIM
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carrier Proteins/metabolism
MH  - Cells, Cultured
MH  - Cholesterol/pharmacology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - *Heat-Shock Proteins
MH  - Humans
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Macrophages/drug effects/*enzymology
MH  - Male
MH  - Microscopy, Immunoelectron
MH  - Middle Aged
MH  - Molecular Chaperones/metabolism
MH  - Sterol O-Acyltransferase/*metabolism
MH  - Tissue Distribution
PMC - PMC1868616
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - S0002-9440(10)64723-2 [pii]
AID - 10.1016/S0002-9440(10)64723-2 [doi]
PST - ppublish
SO  - Am J Pathol. 2000 Jan;156(1):227-36. doi: 10.1016/S0002-9440(10)64723-2.