PMID- 10623634
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 1
DP  - 2000 Jan 7
TI  - NXP-1, a human protein related to Rad21/Scc1/Mcd1, is a component of the nuclear 
      matrix.
PG  - 418-22
AB  - Nuclear matrix is a complex intranuclear network supposed to be involved in the
      various nuclear functions. In order to identify the nuclear matrix proteins, we
      isolated a cDNA clone from a human placenta cDNA library. This clone was
      partially represented a known cDNA clone HA1237. HA1237 encoded a 631-amino-acid 
      peptide, which we designated NXP-1. NXP-1 was related to yeast Rad21/Scc1/Mcd1,
      Xenopus XRAD21, and mouse PW29, and identical with HR21spA isolated from a human 
      testis cDNA library. We developed a polyclonal antibody to the purified NXP-1
      bacterially expressed as a fusion protein with GST. Western blot analysis with
      anti-NXP-1 polyclonal antibody showed nuclear matrix localization of NXP-1 in
      HeLa cells. Indirect immunofluorescence staining also showed nuclear and nuclear 
      matrix localization of the NXP-1. Results of in vitro binding assays employing
      nuclear matrix preparations indicated that the N-terminal region (16-128 amino
      acid) of NXP-1 has an important role in nuclear matrix distribution.
CI  - Copyright 2000 Academic Press.
FAU - Sadano, H
AU  - Sadano H
AD  - Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo, 678-1201,
      Japan.
FAU - Sugimoto, H
AU  - Sugimoto H
FAU - Sakai, F
AU  - Sakai F
FAU - Nomura, N
AU  - Nomura N
FAU - Osumi, T
AU  - Osumi T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (MCD1 protein, S cerevisiae)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RAD21 protein, human)
RN  - 0 (Rad21 protein, Xenopus)
RN  - 0 (Rad21 protein, mouse)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Xenopus Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis Regulatory Proteins
MH  - Cell Cycle Proteins/*chemistry
MH  - Cell Nucleus/*metabolism
MH  - Chromosomal Proteins, Non-Histone
MH  - DNA-Binding Proteins/*chemistry/*genetics/metabolism
MH  - Erythroid-Specific DNA-Binding Factors
MH  - Female
MH  - Gene Library
MH  - HeLa Cells
MH  - Humans
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nuclear Matrix/*metabolism
MH  - Nuclear Proteins/*chemistry/*genetics/metabolism
MH  - Phosphoproteins/*chemistry
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Rats
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Saccharomyces cerevisiae/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Deletion
MH  - Testis/metabolism
MH  - Transcription Factors/*chemistry/*genetics/metabolism
MH  - Xenopus
MH  - *Xenopus Proteins
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - 10.1006/bbrc.1999.1969 [doi]
AID - S0006-291X(99)91969-1 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 7;267(1):418-22. doi:
      10.1006/bbrc.1999.1969.