PMID- 10623627
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20071114
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 1
DP  - 2000 Jan 7
TI  - Lens epithelium-derived growth factor: effects on growth and survival of lens
      epithelial cells, keratinocytes, and fibroblasts.
PG  - 373-81
AB  - We isolated a clone encoding a protein from a human lens epithelial cell (LEC)
      cDNA library with antibody (Ab) from a cataract patient and named it "lens
      epithelium-derived growth factor" (LEDGF). LEDGF is found to be identical to p75,
      a coactivator of both transcription (1) and pre-mRNA splicing (2). In serum-free 
      medium LEDGF stimulated growth of LECs, cos7 cells, skin fibroblasts, and
      keratinocytes, and prolonged cell survival. Without LEDGF, the aforementioned
      cells did not survive. Also in serum-free medium, Ab to LEDGF neutralizing LEDGF 
      blocked cell growth and caused cell death. Thus, LEDGF, a regulatory factor, may 
      play an important role for growth and survival of a wide range of cell types.
CI  - Copyright 2000 Academic Press.
FAU - Singh, D P
AU  - Singh DP
AD  - Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts,
      02115, USA.
FAU - Ohguro, N
AU  - Ohguro N
FAU - Kikuchi, T
AU  - Kikuchi T
FAU - Sueno, T
AU  - Sueno T
FAU - Reddy, V N
AU  - Reddy VN
FAU - Yuge, K
AU  - Yuge K
FAU - Chylack, L T Jr
AU  - Chylack LT Jr
FAU - Shinohara, T
AU  - Shinohara T
LA  - eng
GR  - R01 EY-00484/EY/NEI NIH HHS/United States
GR  - R01 EY-05230/EY/NEI NIH HHS/United States
GR  - R01 EY-12015/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (lens epithelium-derived growth factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Cell Division/drug effects
MH  - Cell Survival/drug effects
MH  - Cloning, Molecular
MH  - Epithelial Cells/cytology/drug effects
MH  - Fibroblasts/cytology/drug effects
MH  - Gene Library
MH  - Growth Substances/chemistry/genetics/*pharmacology/*physiology
MH  - Humans
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Keratinocytes/*cytology/drug effects
MH  - Lens, Crystalline/*cytology/drug effects
MH  - Mice
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/chemistry/pharmacology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 2000/01/07 09:00
MHDA- 2001/03/28 10:01
CRDT- 2000/01/07 09:00
PHST- 2000/01/07 09:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 2000/01/07 09:00 [entrez]
AID - 10.1006/bbrc.1999.1979 [doi]
AID - S0006-291X(99)91979-4 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 7;267(1):373-81. doi:
      10.1006/bbrc.1999.1979.