PMID- 10623621
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 1
DP  - 2000 Jan 7
TI  - A human common nuclear matrix protein homologous to eukaryotic translation
      initiation factor 4A.
PG  - 339-44
AB  - Amino acid sequencing and mass spectrometry revealed identity of a human nuclear 
      matrix protein, termed hNMP 265, with a predicted protein of gene KIAA0111.
      Two-dimensional electrophoresis and Northern hybridization showed the protein to 
      ubiquitously occur in various human cell types. Exhibiting DEAD-box motifs
      characteristic for RNA helicases, hNMP 265 is highly similar to the human
      initiation factors eIF4A-I and -II. On the other hand, hNMP 265 greatly differs
      from the initiation factors by a N-terminal sequence rich in charged amino acids.
      Sequence searches and alignments indicate proteins related to hNMP 265 in other
      eukaryotes. Chimeras between hNMP 265 and green fluorescence protein or hapten
      appeared as speckles in extranucleolar regions in the nucleus, but not in the
      cytoplasm. Experiments with tagged deletion mutants indicated that the N-terminal
      amino acid sequence is necessary for nuclear localization. A putative role of
      hNMP 265 in pre-mRNA processing is discussed.
CI  - Copyright 2000 Academic Press.
FAU - Holzmann, K
AU  - Holzmann K
AD  - Institute of Tumor Biology-Cancer Research, University of Vienna, Borschkegasse
      8a, Vienna, A-1090, Austria.
FAU - Gerner, C
AU  - Gerner C
FAU - Poltl, A
AU  - Poltl A
FAU - Schafer, R
AU  - Schafer R
FAU - Obrist, P
AU  - Obrist P
FAU - Ensinger, C
AU  - Ensinger C
FAU - Grimm, R
AU  - Grimm R
FAU - Sauermann, G
AU  - Sauermann G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.7.- (Eukaryotic Initiation Factor-4A)
RN  - EC 3.6.1.- (EIF4A3 protein, human)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - DEAD-box RNA Helicases
MH  - Eukaryotic Initiation Factor-4A
MH  - HeLa Cells
MH  - Humans
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Nuclear Matrix/*metabolism
MH  - Nuclear Proteins/*chemistry/*genetics/isolation & purification
MH  - Peptide Initiation Factors/*chemistry
MH  - Rats
MH  - Recombinant Fusion Proteins/biosynthesis/chemistry
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - 10.1006/bbrc.1999.1973 [doi]
AID - S0006-291X(99)91973-3 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 7;267(1):339-44. doi:
      10.1006/bbrc.1999.1973.