PMID- 10623583
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20071114
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 1
DP  - 2000 Jan 7
TI  - Methylation and downregulated expression of mac25/insulin-like growth factor
      binding protein-7 is associated with liver tumorigenesis in SV40T/t antigen
      transgenic mice, screened by restriction landmark genomic scanning for
      methylation (RLGS-M).
PG  - 109-17
AB  - Restriction landmark genomic scanning for methylation (RLGS-M) was used to detect
      alterations in DNA methylation associated with murine SV40 T/t antigen-induced
      hepatocarcinogenesis. An altered locus/spot (S130) was cloned and found to
      correspond to sequences in the 5' flanking region and 5' portion of the cDNA for 
      the murine mac25/insulin-like growth factor binding protein-7 (Igfbp-7) gene.
      IGFBPs are believed to be capable of binding insulin, Igf1, and Igf2 and
      modulating mitogenic effects. Previous studies have shown that Igf2 has an
      important role in promoting liver tumorigenesis. Quantitative PCR was used to
      access the methylation status of the NotI site just 5' to the coding region and
      the expression level of the mac25/igfbp-7 gene. The results indicated that the
      degree of methylation was inversely related to the expression level and is
      consistent with a role for DNA methylation in silencing mac25/Igfbp-7 gene
      expression and function for mac25/Igfbp-7 as a tumor suppressor gene.
CI  - Copyright 2000 Academic Press.
FAU - Komatsu, S
AU  - Komatsu S
AD  - Core Research for Evolutional Science and Technology (CREST) of Japan Science and
      Technology Corporation, Tsukuba Life Science Center, Tsukuba, Ibaraki, Japan.
FAU - Okazaki, Y
AU  - Okazaki Y
FAU - Tateno, M
AU  - Tateno M
FAU - Kawai, J
AU  - Kawai J
FAU - Konno, H
AU  - Konno H
FAU - Kusakabe, M
AU  - Kusakabe M
FAU - Yoshiki, A
AU  - Yoshiki A
FAU - Muramatsu, M
AU  - Muramatsu M
FAU - Held, W A
AU  - Held WA
FAU - Hayashizaki, Y
AU  - Hayashizaki Y
LA  - eng
GR  - 5P3CA16056/CA/NCI NIH HHS/United States
GR  - CA68612/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, Polyomavirus Transforming)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Insulin-Like Growth Factor Binding Proteins)
RN  - 0 (insulin-like growth factor binding protein-related protein 1)
SB  - IM
MH  - Animals
MH  - Antigens, Polyomavirus Transforming/*genetics
MH  - Base Sequence
MH  - Carrier Proteins/*genetics
MH  - *DNA Methylation
MH  - DNA, Complementary
MH  - *Gene Expression Regulation
MH  - Gene Library
MH  - Humans
MH  - *Insulin-Like Growth Factor Binding Proteins
MH  - Liver Neoplasms/*genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Muridae
MH  - Restriction Mapping
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Simian virus 40/*genetics
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - 10.1006/bbrc.1999.1937 [doi]
AID - S0006-291X(99)91937-X [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 7;267(1):109-17. doi:
      10.1006/bbrc.1999.1937.