PMID- 10623476
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20161124
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 254
IP  - 1
DP  - 2000 Jan 10
TI  - Isolation and characterization of monoclonal antibodies directed against subunits
      of human RNA polymerases I, II, and III.
PG  - 163-72
AB  - Human nuclei contain three different RNA polymerases: polymerases I, II, and III.
      Each polymerase is a multi-subunit enzyme with 12-17 subunits. The localization
      of these subunits is limited by the paucity of antibodies suitable for
      immunofluorescence. We now describe eight different monoclonal antibodies that
      react specifically with RPB6 (also known as RPA20, RPB14.4, or RPC20), RPB8
      (RPA18, RPB17, or RPC18), RPC32, or RPC39 and which are suitable for such
      studies. Each antibody detects one specific band in immunoblots of nuclear
      extracts; each also immunoprecipitates large complexes containing many other
      subunits. When used for immunofluorescence, antibodies against the subunits
      shared by all three polymerases (i.e., RPB6, RPB8) gave a few bright foci in
      nucleoli and nucleoplasm, as well as many fainter nucleoplasmic foci; all the
      bright foci were generally distinct from speckles containing Sm antigen.
      Antibodies against the two subunits found only in polymerase III (i.e., RPC32,
      RPC39) gave a few bright and many faint nucleoplasmic foci, but no nucleolar
      foci. Growth in two transcriptional inhibitors-5,
      6-dichloro-1-beta-d-ribofuranosylbenzimidazole and actinomycin D-led to the
      redistribution of each subunit in a characteristic manner.
CI  - Copyright 2000 Academic Press.
FAU - Jones, E
AU  - Jones E
AD  - Sir William Dunn School of Pathology, University of Oxford, South Parks Road,
      Oxford, OX1 3RE, United Kingdom.
FAU - Kimura, H
AU  - Kimura H
FAU - Vigneron, M
AU  - Vigneron M
FAU - Wang, Z
AU  - Wang Z
FAU - Roeder, R G
AU  - Roeder RG
FAU - Cook, P R
AU  - Cook PR
LA  - eng
GR  - CA42567/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Autoantigens)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 0 (snRNP Core Proteins)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 53-85-0 (Dichlororibofuranosylbenzimidazole)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 2.7.7.6 (RNA Polymerase I)
RN  - EC 2.7.7.6 (RNA Polymerase III)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology/isolation & purification
MH  - Autoantigens/metabolism
MH  - Dactinomycin/pharmacology
MH  - Dichlororibofuranosylbenzimidazole/pharmacology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Mice
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - RNA Polymerase I/drug effects/*immunology/metabolism
MH  - RNA Polymerase II/drug effects/*immunology/metabolism
MH  - RNA Polymerase III/drug effects/*immunology/metabolism
MH  - *Ribonucleoproteins, Small Nuclear
MH  - snRNP Core Proteins
EDAT- 2000/01/07 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/07 09:00
PHST- 2000/01/07 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/07 09:00 [entrez]
AID - 10.1006/excr.1999.4739 [doi]
AID - S0014-4827(99)94739-0 [pii]
PST - ppublish
SO  - Exp Cell Res. 2000 Jan 10;254(1):163-72. doi: 10.1006/excr.1999.4739.