PMID- 10622721
OWN - NLM
STAT- MEDLINE
DCOM- 20000202
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 462
IP  - 3
DP  - 1999 Dec 3
TI  - cDNA cloning, expression and characterization of human prostaglandin F synthase.
PG  - 335-40
AB  - A cDNA clone of prostaglandin F synthase (PGFS) was isolated from human lung by
      using cDNA of bovine lung-type PGFS as a probe and its protein expressed in
      Escherichia coli was purified to apparent homogeneity. The human PGFS catalyzed
      the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and
      the oxidation of 9alpha,11beta-PGF2 to PGD2. The kcat/Km values for PGD2 and
      9alpha,11beta-PGF2 were 21000 and 1800 min(-1) mM(-1), respectively, indicating
      that the catalytic efficiency for PGD2 and 9alpha,11beta-PGF2 was the highest
      among the various substrates, except for PQ. The PGFS activity in the cytosol of 
      human lung was completely absorbed with antihuman PGFS antiserum. Moreover, mRNA 
      of PGFS was expressed in peripheral blood lymphocytes and the expression in
      lymphocytes was markedly suppressed by the T cell mitogen concanavalin A. These
      results support the notion that human PGFS plays an important role in the
      pathogenesis of allergic diseases such as asthma.
FAU - Suzuki-Yamamoto, T
AU  - Suzuki-Yamamoto T
AD  - Department of Anatomy and Cell Biology, School of Medicine, The University of
      Tokushima, Kuramoto, Japan.
FAU - Nishizawa, M
AU  - Nishizawa M
FAU - Fukui, M
AU  - Fukui M
FAU - Okuda-Ashitaka, E
AU  - Okuda-Ashitaka E
FAU - Nakajima, T
AU  - Nakajima T
FAU - Ito, S
AU  - Ito S
FAU - Watanabe, K
AU  - Watanabe K
LA  - eng
SI  - GENBANK/AB018580
SI  - GENBANK/AB028065
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.1.1.- (Hydroxyprostaglandin Dehydrogenases)
RN  - EC 1.1.1.188 (prostaglandin-F synthase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cloning, Molecular
MH  - Culture Techniques
MH  - DNA, Complementary/metabolism
MH  - Escherichia coli/metabolism
MH  - Gene Library
MH  - Humans
MH  - Hydroxyprostaglandin Dehydrogenases/biosynthesis/*genetics/metabolism
MH  - Kinetics
MH  - Lung/*enzymology
MH  - Lymphocytes/enzymology
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2000/01/06 00:00
MHDA- 2000/01/06 00:01
CRDT- 2000/01/06 00:00
PHST- 2000/01/06 00:00 [pubmed]
PHST- 2000/01/06 00:01 [medline]
PHST- 2000/01/06 00:00 [entrez]
AID - S0014-5793(99)01551-3 [pii]
AID - 10.1016/s0014-5793(99)01551-3 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Dec 3;462(3):335-40. doi: 10.1016/s0014-5793(99)01551-3.