PMID- 10621974
OWN - NLM
STAT- MEDLINE
DCOM- 20000211
LR  - 20191103
IS  - 0962-8819 (Print)
IS  - 0962-8819 (Linking)
VI  - 8
IP  - 4
DP  - 1999 Aug
TI  - Conditional gene targeting in macrophages and granulocytes using LysMcre mice.
PG  - 265-77
AB  - Conditional mutagenesis in mice has recently been made possible through the
      combination of gene targeting techniques and site-directed mutagenesis, using the
      bacteriophage P1-derived Cre/loxP recombination system. The versatility of this
      approach depends on the availability of mouse mutants in which the recombinase
      Cre is expressed in the appropriate cell lineages or tissues. Here we report the 
      generation of mice that express Cre in myeloid cells due to targeted insertion of
      the cre cDNA into their endogenous M lysozyme locus. In double mutant mice
      harboring both the LysMcre allele and one of two different loxP-flanked target
      genes tested, a deletion efficiency of 83-98% was determined in mature
      macrophages and near 100% in granulocytes. Partial deletion (16%) could be
      detected in CD11c+ splenic dendritic cells which are closely related to the
      monocyte/macrophage lineage. In contrast, no significant deletion was observed in
      tail DNA or purified T and B cells. Taken together, LysMcre mice allow for both
      specific and highly efficient Cre-mediated deletion of loxP-flanked target genes 
      in myeloid cells.
FAU - Clausen, B E
AU  - Clausen BE
AD  - Institute for Genetics, University of Cologne, Germany.
FAU - Burkhardt, C
AU  - Burkhardt C
FAU - Reith, W
AU  - Reith W
FAU - Renkawitz, R
AU  - Renkawitz R
FAU - Forster, I
AU  - Forster I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (RFX5 protein, human)
RN  - 0 (Regulatory Factor X Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (DNA Polymerase beta)
RN  - EC 2.7.7.- (Integrases)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Blotting, Southern
MH  - DNA Polymerase beta/genetics
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Gene Deletion
MH  - *Gene Targeting
MH  - Granulocytes/*metabolism
MH  - Histocompatibility Antigens Class II/genetics/metabolism
MH  - Integrases/*genetics/metabolism
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Muramidase/*genetics/metabolism
MH  - Regulatory Factor X Transcription Factors
MH  - *Viral Proteins
EDAT- 2000/01/06 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/06 09:00
PHST- 2000/01/06 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/06 09:00 [entrez]
AID - 10.1023/a:1008942828960 [doi]
PST - ppublish
SO  - Transgenic Res. 1999 Aug;8(4):265-77. doi: 10.1023/a:1008942828960.