PMID- 10620804
OWN - NLM
STAT- MEDLINE
DCOM- 20000306
LR  - 20131121
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 2
IP  - 1
DP  - 2000 Jan
TI  - Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new 
      aspects of centrosome structure and function.
PG  - 30-5
AB  - The centrosome organizes microtubules, which are made up of alpha-tubulin and
      beta-tubulin, and contains centrosome-bound gamma-tubulin, which is involved in
      microtubule nucleation. Here we identify two new human tubulins and show that
      they are associated with the centrosome. One is a homologue of the Chlamydomonas 
      delta-tubulin Uni3, and the other is a new tubulin, which we have named
      epsilon-tubulin. Localization of delta-tubulin and epsilon-tubulin to the
      centrosome is independent of microtubules, and the patterns of localization are
      distinct from each other and from that of gamma-tubulin. Delta-tubulin is found
      in association with the centrioles, whereas epsilon-tubulin localizes to the
      pericentriolar material. epsilon-Tubulin exhibits a cell-cycle-specific pattern
      of localization, first associating with only the older of the centrosomes in a
      newly duplicated pair and later associating with both centrosomes.
      epsilon-Tubulin thus distinguishes the old centrosome from the new at the level
      of the pericentriolar material, indicating that there may be a centrosomal
      maturation event that is marked by the recruitment of epsilon-tubulin.
FAU - Chang, P
AU  - Chang P
AD  - Department of Biological Sciences, Stanford University, Stanford, California
      94305-5020, USA.
FAU - Stearns, T
AU  - Stearns T
LA  - eng
SI  - GENBANK/AF201333
SI  - GENBANK/AF201334
GR  - GM52032/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Tubulin)
RN  - SH1WY3R615 (Nocodazole)
SB  - IM
CIN - Nat Cell Biol. 2000 Jan;2(1):E3-4. PMID: 10620810
MH  - Antineoplastic Agents/pharmacology
MH  - Cell Cycle/physiology
MH  - Cells, Cultured
MH  - Centrosome/*chemistry/*physiology
MH  - Humans
MH  - Microtubules/chemistry/drug effects/physiology
MH  - Molecular Sequence Data
MH  - Nocodazole/pharmacology
MH  - Sequence Homology, Amino Acid
MH  - Tubulin/analysis/genetics/immunology
EDAT- 2000/01/06 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/01/06 09:00
PHST- 2000/01/06 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/01/06 09:00 [entrez]
AID - 10.1038/71350 [doi]
PST - ppublish
SO  - Nat Cell Biol. 2000 Jan;2(1):30-5. doi: 10.1038/71350.