PMID- 10620799
OWN - NLM
STAT- MEDLINE
DCOM- 20000306
LR  - 20071114
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 2
IP  - 1
DP  - 2000 Jan
TI  - Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and promotes
      apoptosis.
PG  - 1-6
AB  - DNA damage induces apoptosis through a signalling pathway that can be suppressed 
      by the BCL-2 protein, but the mechanism by which DNA damage does this is unknown.
      Here, using yeast two-hybrid and co-immunoprecipitation studies, we show that
      RAD9, a human protein involved in the control of a cell-cycle checkpoint,
      interacts with the anti-apoptotic Bcl-2-family proteins BCL-2 and BCL-x L, but
      not with the pro-apoptotic BAX and BAD. When overexpressed in mammalian cells,
      RAD9 induces apoptosis that can be blocked by BCL-2 or BCL-x L. Conversely,
      antisense RAD9 RNA suppresses cell death induced by methyl methanesulphonate.
      These findings indicate that RAD9 may have a new role in regulating apoptosis
      after DNA damage, in addition to its previously described checkpoint-control and 
      other radioresistance-promoting functions.
FAU - Komatsu, K
AU  - Komatsu K
AD  - Drug Discovery Program, H. Lee Moffitt Cancer Center and Research Institute,
      Department of Pharmacology and Therapeutics, University of South Florida College 
      of Medicine, 12902 Magnolia Drive, Tampa, Florida 33612, USA.
FAU - Miyashita, T
AU  - Miyashita T
FAU - Hang, H
AU  - Hang H
FAU - Hopkins, K M
AU  - Hopkins KM
FAU - Zheng, W
AU  - Zheng W
FAU - Cuddeback, S
AU  - Cuddeback S
FAU - Yamada, M
AU  - Yamada M
FAU - Lieberman, H B
AU  - Lieberman HB
FAU - Wang, H G
AU  - Wang HG
LA  - eng
GR  - CA82197-01/CA/NCI NIH HHS/United States
GR  - GM52493/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (BCL2L1 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Antisense)
RN  - 0 (bcl-X Protein)
RN  - 139691-42-2 (rad9 protein)
RN  - 36015-30-2 (Propidium)
SB  - IM
CIN - Nat Cell Biol. 2000 Jan;2(1):E6. PMID: 10620812
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Cell Cycle Proteins/analysis/genetics/metabolism
MH  - Cell Nucleus/chemistry
MH  - Cell Survival/genetics
MH  - Flow Cytometry
MH  - Humans
MH  - Indicators and Reagents
MH  - Mammals
MH  - Molecular Sequence Data
MH  - Plasmids
MH  - Propidium
MH  - Proto-Oncogene Proteins c-bcl-2/analysis/genetics/*metabolism
MH  - RNA, Antisense/pharmacology
MH  - Schizosaccharomyces/*cytology/*genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Signal Transduction/physiology
MH  - Transfection
MH  - Two-Hybrid System Techniques
MH  - bcl-X Protein
EDAT- 2000/01/06 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/01/06 09:00
PHST- 2000/01/06 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/01/06 09:00 [entrez]
AID - 10.1038/71316 [doi]
PST - ppublish
SO  - Nat Cell Biol. 2000 Jan;2(1):1-6. doi: 10.1038/71316.