PMID- 10620492
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 345 Pt 2
DP  - 2000 Jan 15
TI  - Identification of structurally important domains of lipid phosphate
      phosphatase-1: implications for its sites of action.
PG  - 181-4
AB  - Lipid phosphate phosphatase-1 (LPP-1) dephosphorylates exogenous
      lysophosphatidate and thereby regulates the activation of lysophosphatidate
      receptors and cell division. Mutation of seven amino acids in three conserved
      domains of mouse LPP-1 abolished its activity. A glycosylation site was
      demonstrated between conserved Domains 1 and 2. LPP-1 is expressed in the plasma 
      membrane, and the present results demonstrate the active site to be located on
      the outer surface.
FAU - Zhang, Q X
AU  - Zhang QX
AD  - Department of Biochemistry, Lipid and Lipoprotein Research Group, University of
      Alberta, 357 Heritage Medical Research Centre, Edmonton, Alberta, Canada T6G 2S2.
FAU - Pilquil, C S
AU  - Pilquil CS
FAU - Dewald, J
AU  - Dewald J
FAU - Berthiaume, L G
AU  - Berthiaume LG
FAU - Brindley, D N
AU  - Brindley DN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Lysophospholipids)
RN  - 0 (Membrane Proteins)
RN  - EC 3.1.3.- (lipid phosphate phosphatase)
RN  - EC 3.1.3.4 (Phosphatidate Phosphatase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Catalytic Domain
MH  - Cell Membrane/enzymology
MH  - Cells, Cultured
MH  - Conserved Sequence
MH  - Fibroblasts/cytology
MH  - Glycosylation
MH  - Lysophospholipids/*metabolism
MH  - Membrane Proteins
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Phosphatidate Phosphatase/genetics/*metabolism
MH  - Precipitin Tests
MH  - Protein Processing, Post-Translational
MH  - Rats
PMC - PMC1220744
EDAT- 2000/01/06 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/06 09:00
PHST- 2000/01/06 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/06 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 2000 Jan 15;345 Pt 2:181-4.