PMID- 10620008
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20191103
IS  - 1356-9597 (Print)
IS  - 1356-9597 (Linking)
VI  - 4
IP  - 11
DP  - 1999 Nov
TI  - Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli
      mutagenesis protein dinB.
PG  - 607-18
AB  - BACKGROUND: The Escherichia coli dinB gene is an SOS gene known to be required
      for lambda phage untargeted mutagenesis. When over-expressed, it exhibits a
      potent mutagenic activity without any exogenous treatment to damage DNA.
      Frameshift mutations at a run of identical bases are most enhanced. The product
      DinB is structurally related to the E. coli UmuC protein and the Saccharomyces
      cerevisiae Rev1 and Rad30 proteins, all of which are shown to be involved in
      bypass synthesis at a DNA lesion. RESULTS: We have cloned and sequenced human and
      mouse cDNAs encoding a DinB homologue. Their products are highly similar to DinB 
      and less similar to UmuC, Rev1 or Rad30, and hence the genes were named DINB1 for
      human and Dinb1 for mouse. Both genes were expressed most abundantly in testis.
      Transient expression of the mouse cDNA in cultured mouse cells resulted in a
      nearly 10-fold increase in the incidence of point mutations, among which about
      30% were frameshift mutations. CONCLUSIONS: The above results suggest that a
      mutagenic mechanism, a so-called untargeted type, also operates in mammalian
      cells. Taken together with recent findings that human cells have multiple DNA
      polymerases for translesion synthesis which are homologous to the S. cerevisiae
      Rev3 and Rad30 proteins, our results imply that multiple mutagenic pathways are
      conserved from bacteria to higher eukaryotes.
FAU - Ogi, T
AU  - Ogi T
AD  - Institute for Virus Research, Kyoto University, Kawahara-machi, Sakyo-ku, Kyoto
      606-8507, Japan.
FAU - Kato, T Jr
AU  - Kato T Jr
FAU - Kato, T
AU  - Kato T
FAU - Ohmori, H
AU  - Ohmori H
LA  - eng
SI  - GENBANK/AB027563
SI  - GENBANK/AB027564
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (DinB protein, E coli)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Proteins)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
RN  - EC 2.7.7.7 (POLK protein, human)
RN  - EC 2.7.7.7 (Polk protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Proteins/chemistry/*genetics
MH  - Cells, Cultured
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA Mutational Analysis
MH  - DNA, Complementary/analysis
MH  - *DNA-Directed DNA Polymerase
MH  - Escherichia coli/*genetics
MH  - *Escherichia coli Proteins
MH  - Humans
MH  - Hypoxanthine Phosphoribosyltransferase/genetics
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis/*physiology
MH  - Proteins/chemistry/*genetics
MH  - Sequence Analysis, DNA
MH  - *Sequence Homology, Amino Acid
EDAT- 2000/01/05 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/05 09:00
PHST- 2000/01/05 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/05 09:00 [entrez]
AID - gtc289 [pii]
AID - 10.1046/j.1365-2443.1999.00289.x [doi]
PST - ppublish
SO  - Genes Cells. 1999 Nov;4(11):607-18. doi: 10.1046/j.1365-2443.1999.00289.x.