PMID- 10619843
OWN - NLM
STAT- MEDLINE
DCOM- 20000223
LR  - 20081120
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 19
IP  - 1
DP  - 2000 Jan 4
TI  - The VacA toxin of Helicobacter pylori identifies a new intermediate
      filament-interacting protein.
PG  - 48-56
AB  - The VacA toxin produced by Helicobacter pylori acts inside cells and induces the 
      formation of vacuoles arising from late endosomal/lysosomal compartments. Using
      VacA as bait in a yeast two-hybrid screening of a HeLa cell library, we have
      identified a novel protein of 54 kDa (VIP54), which interacts specifically with
      VacA, as indicated by co-immunoprecipitation and binding experiments. VIP54 is
      expressed in cultured cells and many tissues, with higher expression in the
      brain, muscle, kidney and liver. Confocal immunofluorescence microscopy with
      anti-VIP54 affinity- purified antibodies shows a fibrous pattern typical of
      intermediate filaments. Double label immunofluorescence performed on various cell
      lines with antibodies specific to different intermediate filament proteins
      revealed that VIP54 largely co-distributes with vimentin. In contrast to known
      intermediate filament proteins, VIP54 is predicted to contain approximately 50%
      of helical segments, but no extended coiled-coil regions. The possible
      involvement of this novel protein in interactions between intermediate filaments 
      and late endosomal compartments is discussed.
FAU - de Bernard, M
AU  - de Bernard M
AD  - Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Universita di
      Padova, Via G.Colombo 3, 35121 Padova, Italy. marina@civ.bio.unipd.it
FAU - Moschioni, M
AU  - Moschioni M
FAU - Napolitani, G
AU  - Napolitani G
FAU - Rappuoli, R
AU  - Rappuoli R
FAU - Montecucco, C
AU  - Montecucco C
LA  - eng
SI  - GENBANK/AA840455
SI  - GENBANK/AC007439
SI  - GENBANK/AI158261
SI  - GENBANK/AJ242655
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cytotoxins)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (VIP54 protein, Helicobacter pylori)
RN  - 0 (VacA protein, Helicobacter pylori)
RN  - 0 (Vimentin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Astrocytes/chemistry
MH  - Bacterial Proteins/*metabolism
MH  - Bacterial Toxins/*metabolism
MH  - Cell Line
MH  - Cytotoxins/metabolism
MH  - Dogs
MH  - HeLa Cells
MH  - Helicobacter pylori/*metabolism
MH  - Humans
MH  - Intermediate Filament Proteins/immunology/*isolation & purification/metabolism
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Neuroblastoma/chemistry
MH  - Neurons/chemistry
MH  - Tumor Cells, Cultured
MH  - Vacuoles/metabolism
MH  - Vimentin/isolation & purification
PMC - PMC1171776
EDAT- 2000/01/05 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/05 09:00
PHST- 2000/01/05 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/05 09:00 [entrez]
AID - 10.1093/emboj/19.1.48 [doi]
PST - ppublish
SO  - EMBO J. 2000 Jan 4;19(1):48-56. doi: 10.1093/emboj/19.1.48.