PMID- 10619026
OWN - NLM
STAT- MEDLINE
DCOM- 20000114
LR  - 20131121
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 4
IP  - 5
DP  - 1999 Nov
TI  - The structural basis of Rho effector recognition revealed by the crystal
      structure of human RhoA complexed with the effector domain of PKN/PRK1.
PG  - 793-803
AB  - The small G protein Rho has emerged as a key regulator of cellular events
      involving cytoskeletal reorganization. Here we report the 2.2 A crystal structure
      of RhoA bound to an effector domain of protein kinase PKN/PRK1. The structure
      reveals the antiparallel coiled-coil finger (ACC finger) fold of the effector
      domain that binds to the Rho specificity-determining regions containing switch I,
      beta strands B2 and B3, and the C-terminal alpha helix A5, predominantly by
      specific hydrogen bonds. The ACC finger fold is distinct from those for other
      small G proteins and provides evidence for the diverse ways of effector
      recognition. Sequence analysis based on the structure suggests that the ACC
      finger fold is widespread in Rho effector proteins.
FAU - Maesaki, R
AU  - Maesaki R
AD  - Division of Structural Biology, Nara Institute of Science and Technology, Japan.
FAU - Ihara, K
AU  - Ihara K
FAU - Shimizu, T
AU  - Shimizu T
FAU - Kuroda, S
AU  - Kuroda S
FAU - Kaibuchi, K
AU  - Kaibuchi K
FAU - Hakoshima, T
AU  - Hakoshima T
LA  - eng
SI  - PDB/1CXZ
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate))
RN  - EC 2.7.1.- (protein kinase N)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Amino Acid Substitution
MH  - Binding Sites
MH  - *Catalytic Domain
MH  - Crystallography, X-Ray
MH  - Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
MH  - Humans
MH  - Hydrogen Bonding
MH  - Leucine Zippers
MH  - Magnesium/metabolism
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Kinase C/*chemistry/*metabolism
MH  - Protein Structure, Secondary
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Structure-Activity Relationship
MH  - rhoA GTP-Binding Protein/*chemistry/genetics/*metabolism
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - S1097-2765(00)80389-5 [pii]
PST - ppublish
SO  - Mol Cell. 1999 Nov;4(5):793-803.