PMID- 10618521
OWN - NLM
STAT- MEDLINE
DCOM- 20000121
LR  - 20061115
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 232
IP  - 1-2
DP  - 1999 Dec 17
TI  - Processing and targeting of granule proteins in human neutrophils.
PG  - 201-10
AB  - Neutrophils contain an assembly of granules destined for regulated secretion,
      each granule type with distinct constituents formed before terminal
      differentiation. The earliest granules are designated azurophil (primary),
      followed in time by specific (secondary), and gelatinase granules as well as
      secretory vesicles. Transcription factors regulate the genes for the granule
      proteins to ensure that expression of the gene products to be stored in different
      organelles is separated in time. Similar to lysosomal enzymes, many granule
      proteins, in particular those of the heterogeneous azurophil granules, are
      trimmed by proteolytic processing into mature proteins. Rodent myeloid cell lines
      have been utilized for research on the processing and targeting of human granule 
      proteins after transfection of cDNA. Results from extensive work on the
      hematopoietic serine proteases of azurophil granules, employing in vitro
      mutagenesis, indicate that both an immature and a mature conformation are
      compatible with targeting for storage in granules. On the other hand, the
      amino-terminal propeptide of myeloperoxidase facilitates both the export from the
      endoplasmic reticulum and targeting for storage in granules. Similarly, targeting
      of defensins rely on an intact propeptide. The proteolytic processing into mature
      granule protein is most commonly a post-sorting event. Mis-sorting of specific
      granule proteins into azurophil or lysosome-like granules can result in premature
      activation and degradation, but represents a potential for manipulating the
      composition and function of neutrophil granules.
FAU - Gullberg, U
AU  - Gullberg U
AD  - Department of Hematology, Research Department 2, E-blocket, University Hospital, 
      S-221 85, Lund, Sweden. urban.gullberg@hematologi.lu.se
FAU - Bengtsson, N
AU  - Bengtsson N
FAU - Bulow, E
AU  - Bulow E
FAU - Garwicz, D
AU  - Garwicz D
FAU - Lindmark, A
AU  - Lindmark A
FAU - Olsson, I
AU  - Olsson I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Cytoplasmic Granules/*metabolism
MH  - Humans
MH  - Neutrophils/*metabolism
MH  - Protein Processing, Post-Translational/*immunology
MH  - Protein Sorting Signals/metabolism
MH  - Proteins/*metabolism
RF  - 57
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - S0022-1759(99)00177-5 [pii]
PST - ppublish
SO  - J Immunol Methods. 1999 Dec 17;232(1-2):201-10.