PMID- 10618498
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 464
IP  - 3
DP  - 1999 Dec 31
TI  - Phosphorylation of the Spi-B transcription factor reduces its intrinsic
      stability.
PG  - 164-8
AB  - The Spi-B transcription factor is an Ets protein expressed in B lymphoid cells
      and closely related to the Spi-1/PU.1 oncoprotein. By mutational analysis, we
      showed that Spi-B is phosphorylated by casein kinase II in vitro on four serine
      residues. Mutation of these four serines to alanines prevented the
      phosphorylation of Spi-B in vivo, increased the ability of Spi-B to transactivate
      expression of a reporter gene and led to a decrease of Spi-B stability. We
      propose that the phosphorylation of Spi-B may participate in the modulation of
      Spi-B functional activity by controlling its intracellular protein level.
FAU - Ray-Gallet, D
AU  - Ray-Gallet D
AD  - INSERM, Unite 248, Institut Curie, 26 rue diUlm, 75248, Paris, France.
FAU - Moreau-Gachelin, F
AU  - Moreau-Gachelin F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - 148350-00-9 (SPIB protein, human)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Casein Kinase II
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Mutation
MH  - Phosphorylation
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - Transcription Factors/*metabolism
MH  - Transcriptional Activation
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - S0014-5793(99)01687-7 [pii]
AID - 10.1016/s0014-5793(99)01687-7 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Dec 31;464(3):164-8. doi: 10.1016/s0014-5793(99)01687-7.