PMID- 10617859
OWN - NLM
STAT- MEDLINE
DCOM- 20000201
LR  - 20190921
IS  - 0022-104X (Print)
IS  - 0022-104X (Linking)
VI  - 286
IP  - 2
DP  - 2000 Feb 1
TI  - Developmental patterns of zygotes from transgenic female mice with elevated
      tissue glutathione.
PG  - 173-80
AB  - Zygotes were collected from transgenic mice overexpressing glutathione synthetase
      to test the hypothesis that such zygotes are more capable of developing in
      suboptimal culture media than zygotes from non-transgenic (control) mice. The
      effects of injection of donor mice with gamma-glutamylcysteinyl ethyl ester
      (gamma-GCE) on embryonic development were also investigated. In addition, the
      effects of genetic background (i.e., transgenic vs. non-transgenic) and injection
      with gamma-GCE on developmental capacity in kSOM were studied after exposure of
      zygotes to diamide (0.01 mM, for 30 min). When cultured in modified Medium 16
      significantly more pronuclear ova from transgenic females reached the morula (M) 
      and early blastocyst (EB) stages than embryos derived from control mice. Genetic 
      background significantly affected the proportions of embryos reaching 4- to
      16-cell, M and EB stages during culture in modified Whitten's medium (WM); more
      zygotes collected from transgenic than from control mice developed. The injection
      of experimental mice with gamma-GCE significantly increased proportions of
      zygotes developing to M and EB stages in WM. Following exposure to diamide and
      subsequent culture in kSOM significantly more zygotes collected from transgenic
      mice reached the 4- to 16-cell stages than those from control females; a
      significant positive effect on developmental capacity was also seen after
      injection of donor mice with gamma-GCE. When cultured in suboptimal conditions,
      zygotes derived from transgenic mice overexpressing glutathione synthetase were
      more capable of developing than zygotes of non-transgenic control females.
      Zygotes from the transgenic mice also exhibited greater capacity to withstand
      toxic exposure to diamide. Present data suggest that commonly known strain
      differences in preimplantational development in vitro may reflect differences in 
      the synthesis and/or metabolism of glutathione. J. Exp. Zool. 286:173-180, 2000.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Rzucidlo, S J
AU  - Rzucidlo SJ
AD  - Department of Physiology and Pharmacology, College of Veterinary Medicine,
      University of Georgia, Athens, Georgia 30602, USA.
FAU - Brackett, B G
AU  - Brackett BG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Zool
JT  - The Journal of experimental zoology
JID - 0375365
RN  - 10465-78-8 (Diamide)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Culture Techniques
MH  - Diamide/pharmacology
MH  - Female
MH  - Glutathione/analysis/*physiology
MH  - Mice
MH  - Mice, Transgenic/*embryology
MH  - Oocytes/chemistry
MH  - Zygote/drug effects/*growth & development
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1002/(SICI)1097-010X(20000201)286:2<173::AID-JEZ9>3.0.CO;2-Q [pii]
AID - 10.1002/(sici)1097-010x(20000201)286:2<173::aid-jez9>3.0.co;2-q [doi]
PST - ppublish
SO  - J Exp Zool. 2000 Feb 1;286(2):173-80. doi:
      10.1002/(sici)1097-010x(20000201)286:2<173::aid-jez9>3.0.co;2-q.