PMID- 10617663
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 1
DP  - 2000 Jan 7
TI  - A mechanism of repression by acute myeloid leukemia-1, the target of multiple
      chromosomal translocations in acute leukemia.
PG  - 651-6
AB  - AML1 is one of the most frequently translocated genes in human leukemia. Here we 
      demonstrate that acute myeloid leukemia-1 (AML-1) (Runx-1) represses
      transcription from a native promoter, p21(Waf1/Cip1). Unexpectedly, this
      repression did not require interactions with the Groucho co-repressor. To define 
      the mechanism of repression, we asked whether other co-repressors could interact 
      with AML-1. We demonstrate that AML-1 interacts with the mSin3 co-repressors.
      Moreover, endogenous AML-1 associated with endogenous mSin3A in mammalian cells. 
      A deletion mutant of AML-1 that did not interact with mSin3A failed to repress
      transcription. The AML-1/mSin3 association suggests a mechanism of repression for
      the chromosomal translocation fusion proteins that disrupt AML-1.
FAU - Lutterbach, B
AU  - Lutterbach B
AD  - Departments of Biochemistry, Vanderbilt University School of Medicine, Nashville,
      Tennessee 37232, USA.
FAU - Westendorf, J J
AU  - Westendorf JJ
FAU - Linggi, B
AU  - Linggi B
FAU - Isaac, S
AU  - Isaac S
FAU - Seto, E
AU  - Seto E
FAU - Hiebert, S W
AU  - Hiebert SW
LA  - eng
GR  - R01-AG13726/AG/NIA NIH HHS/United States
GR  - R01-CA64140/CA/NCI NIH HHS/United States
GR  - R01-CA77274/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Core Binding Factor alpha Subunits)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SIN3 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Acute Disease
MH  - Binding Sites
MH  - Core Binding Factor Alpha 2 Subunit
MH  - Core Binding Factor alpha Subunits
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/*genetics
MH  - DNA-Binding Proteins/metabolism
MH  - Histone Deacetylases
MH  - Leukemia, Myeloid/*genetics
MH  - *Neoplasm Proteins
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - *Proto-Oncogene Proteins
MH  - Repressor Proteins/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Transcription Factors/*metabolism
MH  - *Translocation, Genetic
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1074/jbc.275.1.651 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 7;275(1):651-6. doi: 10.1074/jbc.275.1.651.