PMID- 10617654
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20191210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 1
DP  - 2000 Jan 7
TI  - The copper transport protein Atox1 promotes neuronal survival.
PG  - 580-4
AB  - Atox1, a copper transport protein, was recently identified as a copper-dependent 
      suppressor of oxidative damage in yeast lacking superoxide dismutase. We have
      previously reported that Atox1 in the rat brain is primarily expressed in
      neurons, with the highest levels in distinct neuronal subtypes that are
      characterized by their high levels of metal, like copper, iron, and zinc. In this
      report, we have transfected the Atox1 gene into several neuronal cell lines to
      increase the endogenous level of Atox1 expression and have demonstrated that,
      under conditions of serum starvation and oxidative injury, the transfected
      neurons are significantly protected against this stress. This level of protection
      is comparable with the level of protection seen with copper/zinc superoxide
      dismutase and the anti-apoptotic gene bcl-2 that had been similarly transfected. 
      Furthermore, neuronal cell lines transfected with a mutant Atox1 gene, where the 
      copper binding domain has been modified to prevent metal binding, do not afford
      protection against serum starvation resulting in apoptosis. Therefore, Atox1 is a
      component of the cellular pathways used for protection against oxidative stress.
FAU - Kelner, G S
AU  - Kelner GS
AD  - Department of Molecular Biology, Neurocrine Biosciences, San Diego, California
      92121, USA.
FAU - Lee, M
AU  - Lee M
FAU - Clark, M E
AU  - Clark ME
FAU - Maciejewski, D
AU  - Maciejewski D
FAU - McGrath, D
AU  - McGrath D
FAU - Rabizadeh, S
AU  - Rabizadeh S
FAU - Lyons, T
AU  - Lyons T
FAU - Bredesen, D
AU  - Bredesen D
FAU - Jenner, P
AU  - Jenner P
FAU - Maki, R A
AU  - Maki RA
LA  - eng
SI  - GENBANK/AF127137
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Atox1 protein, rat)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Copper Transport Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Neuropeptides)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Recombinant Proteins)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Transport
MH  - Brain/physiology
MH  - Carrier Proteins/genetics/*metabolism
MH  - *Cation Transport Proteins
MH  - Cell Survival
MH  - Cloning, Molecular
MH  - Copper/*metabolism
MH  - Copper Transport Proteins
MH  - *Molecular Chaperones
MH  - Molecular Sequence Data
MH  - Neurons/*cytology
MH  - Neuropeptides/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1074/jbc.275.1.580 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 7;275(1):580-4. doi: 10.1074/jbc.275.1.580.