PMID- 10617653
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 1
DP  - 2000 Jan 7
TI  - Coregulator small nuclear RING finger protein (SNURF) enhances Sp1- and steroid
      receptor-mediated transcription by different mechanisms.
PG  - 571-9
AB  - The small nuclear RING finger protein SNURF is not only a coactivator in steroid 
      receptor-dependent transcription but also activates transcription from
      steroid-independent promoters. In this work, we show that SNURF, via the RING
      finger domain, enhances protein binding to Sp1 elements/GC boxes and interacts
      and cooperates with Sp1 in transcriptional activation. The activation of androgen
      receptor (AR) function requires regions other than the RING finger of SNURF, and 
      SNURF does not influence binding of AR to cognate DNA elements. The zinc finger
      region (ZFR) together with the hinge region of AR are sufficient for contacting
      SNURF. The nuclear localization signal in the boundary between ZFR and the hinge 
      region participates in the association of AR with SNURF, and a receptor mutant
      lacking the C-terminal part of the bipartite nuclear localization signal shows
      attenuated response to coexpressed SNURF. Some AR ZFR point mutations observed in
      patients with partial androgen insensitivity syndrome or male breast cancer
      impair the interaction of AR with SNURF and also render AR refractory to the
      transcription-activating effect of SNURF. Collectively, SNURF modulates the
      transcriptional activities of androgen receptor and Sp1 via different domains,
      and it may act as a functional link between steroid- and Sp1-regulated
      transcription.
FAU - Poukka, H
AU  - Poukka H
AD  - Department of Physiology, Institute of Biomedicine, FIN-00014 Helsinki, Finland.
FAU - Aarnisalo, P
AU  - Aarnisalo P
FAU - Santti, H
AU  - Santti H
FAU - Janne, O A
AU  - Janne OA
FAU - Palvimo, J J
AU  - Palvimo JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNF4 protein, human)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Androgen-Insensitivity Syndrome/genetics
MH  - Breast Neoplasms, Male/genetics
MH  - DNA-Binding Proteins/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Nuclear Localization Signals
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Point Mutation
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Receptors, Androgen/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Sp1 Transcription Factor/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcription, Genetic
MH  - *Zinc Fingers/genetics
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1074/jbc.275.1.571 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 7;275(1):571-9. doi: 10.1074/jbc.275.1.571.