PMID- 10617623
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 1
DP  - 2000 Jan 7
TI  - Genomic organization and expression pattern of mouse neuroglycan C in the
      cerebellar development.
PG  - 337-42
AB  - Neuroglycan C (NGC) is a membrane-spanning chondroitin sulfate proteoglycan with 
      an epidermal growth factor module that is expressed predominantly in the brain.
      Cloning studies with mouse NGC cDNA revealed the expression of three distinct
      isoforms (NGC-I, -II, and -III) in the brain and revealed that the major isoform 
      showed 94. 3% homology with the rat counterpart. The NGC gene comprised six
      exons, was approximately 17 kilobases in size, and was assigned to mouse
      chromosome band 9F1 by fluorescence in situ hybridization. Western blot analysis 
      demonstrated that, although NGC in the immature cerebellum existed in a
      proteoglycan form, most NGC in the mature cerebellum did not bear chondroitin
      sulfate chain(s), indicating that NGC is a typical part-time proteoglycan.
      Immunohistochemical studies showed that only the Purkinje cells were
      immunopositive in the cerebellum. In the immature Purkinje cells, NGC, probably
      the proteoglycan form, was immunolocalized to the soma and thick dendrites on
      which the climbing fibers formed synapses, not to the thin branches on which the 
      parallel fibers formed synapses. This finding suggests the involvement of NGC in 
      the differential adhesion and synaptogenesis of the climbing and parallel fibers 
      with the Purkinje cell dendrites.
FAU - Aono, S
AU  - Aono S
AD  - Departments of Perinatology, Institute for Developmental Research, Aichi Human
      Service Center, Kasugai, Aichi 480-0392, Japan. saono@inst-hsc.pref.aichi.jp
FAU - Keino, H
AU  - Keino H
FAU - Ono, T
AU  - Ono T
FAU - Yasuda, Y
AU  - Yasuda Y
FAU - Tokita, Y
AU  - Tokita Y
FAU - Matsui, F
AU  - Matsui F
FAU - Taniguchi, M
AU  - Taniguchi M
FAU - Sonta, S
AU  - Sonta S
FAU - Oohira, A
AU  - Oohira A
LA  - eng
SI  - GENBANK/AF133700
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cspg5 protein, mouse)
RN  - 0 (Cspg5 protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proteoglycans)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cerebellum/*growth & development
MH  - Exons
MH  - Gene Library
MH  - Genomic Library
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Membrane Proteins/biosynthesis/chemistry/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Protein Isoforms
MH  - Proteoglycans/biosynthesis/chemistry/*genetics
MH  - Purkinje Cells/chemistry
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1074/jbc.275.1.337 [doi]
AID - S0021-9258(19)52870-8 [pii]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 7;275(1):337-42. doi: 10.1074/jbc.275.1.337.