PMID- 10617617
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 275
IP  - 1
DP  - 2000 Jan 7
TI  - Sphingosine 1-phosphate-induced cell proliferation, survival, and related
      signaling events mediated by G protein-coupled receptors Edg3 and Edg5.
PG  - 288-96
AB  - Sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, 
      and neurite retraction. Contradictory reports propose that S1P acts as either an 
      intracellular second messenger or an extracellular ligand for cell-surface
      receptors. Hence, the precise signaling mechanisms mediating the diverse cellular
      effects of S1P remain to be determined. Here, we investigate whether S1P
      stimulation of cell proliferation, survival, and related signaling events can be 
      mediated by the recently cloned Edg family members of G protein-coupled
      receptors. We observed that S1P treatment significantly increased proliferation
      of HTC4 hepatoma cells stably transfected with human S1P receptor Edg3 or Edg5,
      which was attributable to stimulation of cell growth and inhibition of apoptosis 
      caused by serum starvation. Edg3 and Edg5 transduced S1P-evoked signaling events 
      relevant to cell proliferation and survival, including activation of the ERK/MAP 
      kinases, and immediate-early induction of c-Jun and c-Fos. Trancriptional
      activation of reporter genes for the c-fos promoter and the serum response
      element by Edg3 and Edg5 transfected in Jurkat cells was inhibited by pertussis
      toxin and C3 exoenzyme, implicating G(i/o)- and Rho-dependent pathways. Our data 
      also indicated that Edg3 and Edg5 mediated the serum response element activation 
      through transcriptional factors Elk-1 and serum response factor. Thus, specific G
      protein-coupled receptors Edg3 and Edg5 account for, at least in part,
      S1P-induced cell proliferation, survival, and related signaling events.
FAU - An, S
AU  - An S
AD  - Department of Medicine, University of California, San Francisco, California
      94143-0711, USA. songzhu@itsa.ucsf.edu
FAU - Zheng, Y
AU  - Zheng Y
FAU - Bleu, T
AU  - Bleu T
LA  - eng
SI  - GENBANK/AF034780
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ELK1 protein, human)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Lysophospholipids)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Lysophospholipid)
RN  - 0 (Serum Response Factor)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transcription Factors)
RN  - 0 (ets-Domain Protein Elk-1)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 26993-30-6 (sphingosine 1-phosphate)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gi-Go)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - NGZ37HRE42 (Sphingosine)
SB  - IM
MH  - Cell Division
MH  - Cell Survival
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/metabolism
MH  - GTP-Binding Protein alpha Subunits, Gi-Go
MH  - GTP-Binding Proteins/*metabolism
MH  - Humans
MH  - *I-kappa B Proteins
MH  - *Lysophospholipids
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Molecular Sequence Data
MH  - NF-KappaB Inhibitor alpha
MH  - Nuclear Proteins/metabolism
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - *Receptors, G-Protein-Coupled
MH  - Receptors, Lysophospholipid
MH  - Serum Response Factor
MH  - Signal Transduction
MH  - Sphingosine/*analogs & derivatives/metabolism
MH  - Transcription Factor AP-1/metabolism
MH  - *Transcription Factors
MH  - ets-Domain Protein Elk-1
MH  - rho GTP-Binding Proteins/metabolism
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1074/jbc.275.1.288 [doi]
PST - ppublish
SO  - J Biol Chem. 2000 Jan 7;275(1):288-96. doi: 10.1074/jbc.275.1.288.