PMID- 10617567
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20220330
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 13
IP  - 24
DP  - 1999 Dec 15
TI  - T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification.
PG  - 3185-90
AB  - Wnt3a encodes a signal that is expressed in the primitive streak of the 
      gastrulating mouse embryo and is required for paraxial mesoderm development. In 
      its absence cells adopt ectopic neural fates. Embryos lacking the 
      T-box-containing transcription factors, Brachyury or Tbx6, also lack paraxial 
      mesoderm. Here we show that Brachyury is specifically down-regulated in Wnt3a 
      mutants in cells fated to form paraxial mesoderm. Transgenic analysis of the T 
      promoter identifies T (Brachyury) as a direct transcriptional target of the Wnt 
      signaling pathway. Our results suggest that Wnt3a, signaling via Brachyury, 
      modulates a balance between mesodermal and neural cell fates during gastrulation.
FAU - Yamaguchi, T P
AU  - Yamaguchi TP
AD  - Department of Molecular Biology, Biological Laboratories, Harvard University, 
      Cambridge, Massachusetts 02138 USA.
FAU - Takada, S
AU  - Takada S
FAU - Yoshikawa, Y
AU  - Yoshikawa Y
FAU - Wu, N
AU  - Wu N
FAU - McMahon, A P
AU  - McMahon AP
LA  - eng
GR  - HD30249/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Fetal Proteins)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (Wnt Proteins)
RN  - 0 (Wnt3 Protein)
RN  - 0 (Wnt3A Protein)
RN  - 0 (Wnt3a protein, mouse)
RN  - EC 3.2.1.23 (beta-Galactosidase)
RN  - EQ43SC3GDB (Brachyury protein)
SB  - IM
MH  - Animals
MH  - Embryonic and Fetal Development
MH  - *Fetal Proteins
MH  - Gastrula/*physiology
MH  - Gene Expression Regulation, Developmental
MH  - Heterozygote
MH  - Homozygote
MH  - Mesoderm/*physiology
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Mutagenesis, Site-Directed
MH  - Promoter Regions, Genetic
MH  - Proteins/*genetics/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Signal Transduction
MH  - T-Box Domain Proteins/*genetics/*metabolism
MH  - Wnt Proteins
MH  - Wnt3 Protein
MH  - Wnt3A Protein
MH  - beta-Galactosidase/genetics
PMC - PMC317203
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1101/gad.13.24.3185 [doi]
PST - ppublish
SO  - Genes Dev. 1999 Dec 15;13(24):3185-90. doi: 10.1101/gad.13.24.3185.