PMID- 10617468
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 286
IP  - 5449
DP  - 1999 Dec 24
TI  - Reduced MAP kinase phosphatase-1 degradation after p42/p44MAPK-dependent
      phosphorylation.
PG  - 2514-7
AB  - The mitogen-activated protein (MAP) kinase cascade is inactivated at the level of
      MAP kinase by members of the MAP kinase phosphatase (MKP) family, including
      MKP-1. MKP-1 was a labile protein in CCL39 hamster fibroblasts; its degradation
      was attenuated by inhibitors of the ubiquitin-directed proteasome complex. MKP-1 
      was a target in vivo and in vitro for p42(MAPK) or p44(MAPK), which
      phosphorylates MKP-1 on two carboxyl-terminal serine residues, Serine 359 and
      Serine 364. This phosphorylation did not modify MKP-1's intrinsic ability to
      dephosphorylate p44(MAPK) but led to stabilization of the protein. These results 
      illustrate the importance of regulated protein degradation in the control of
      mitogenic signaling.
FAU - Brondello, J M
AU  - Brondello JM
AD  - Institute of Signaling, Developmental Biology and Cancer Research, CNRS UMR 6543,
      Centre A. Lacassagne, 33 Avenue de Valombrose, Nice 06189, France.
FAU - Pouyssegur, J
AU  - Pouyssegur J
FAU - McKenzie, F R
AU  - McKenzie FR
LA  - eng
GR  - GM26939/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Culture Media)
RN  - 0 (Cysteine Proteinase Inhibitors)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Leupeptins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Nitrophenols)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (Ubiquitins)
RN  - 110044-82-1 (acetylleucyl-leucyl-norleucinal)
RN  - 330-13-2 (nitrophenylphosphate)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 1)
RN  - EC 3.1.3.48 (DUSP1 protein, human)
RN  - EC 3.1.3.48 (Dual Specificity Phosphatase 1)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - GMW67QNF9C (Leucine)
RN  - R76F7856MV (E 64)
SB  - IM
MH  - Animals
MH  - Blood
MH  - *Cell Cycle Proteins
MH  - Cell Division
MH  - Cell Line
MH  - Cricetinae
MH  - Culture Media
MH  - Cysteine Endopeptidases/metabolism
MH  - Cysteine Proteinase Inhibitors/pharmacology
MH  - Dual Specificity Phosphatase 1
MH  - Estradiol/pharmacology
MH  - Humans
MH  - Immediate-Early Proteins/chemistry/*metabolism
MH  - Leucine/analogs & derivatives/pharmacology
MH  - Leupeptins/pharmacology
MH  - MAP Kinase Signaling System
MH  - Mitogen-Activated Protein Kinase 1/*metabolism
MH  - Mitogen-Activated Protein Kinase 3
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Multienzyme Complexes/metabolism
MH  - Mutation
MH  - Nitrophenols/metabolism
MH  - Organophosphorus Compounds/metabolism
MH  - *Phosphoprotein Phosphatases
MH  - Phosphorylation
MH  - Proteasome Endopeptidase Complex
MH  - Protein Phosphatase 1
MH  - Protein Tyrosine Phosphatases/chemistry/*metabolism
MH  - Ubiquitins/metabolism
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 8131 [pii]
AID - 10.1126/science.286.5449.2514 [doi]
PST - ppublish
SO  - Science. 1999 Dec 24;286(5449):2514-7. doi: 10.1126/science.286.5449.2514.