PMID- 10615055
OWN - NLM
STAT- MEDLINE
DCOM- 20000120
LR  - 20220227
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 287
IP  - 5450
DP  - 2000 Jan 7
TI  - Structural basis of Smad2 recognition by the Smad anchor for receptor activation.
PG  - 92-7
AB  - The Smad proteins mediate transforming growth factor-beta (TGFbeta) signaling
      from the transmembrane serine-threonine receptor kinases to the nucleus. The Smad
      anchor for receptor activation (SARA) recruits Smad2 to the TGFbeta receptors for
      phosphorylation. The crystal structure of a Smad2 MH2 domain in complex with the 
      Smad-binding domain (SBD) of SARA has been determined at 2.2 angstrom resolution.
      SARA SBD, in an extended conformation comprising a rigid coil, an alpha helix,
      and a beta strand, interacts with the beta sheet and the three-helix bundle of
      Smad2. Recognition between the SARA rigid coil and the Smad2 beta sheet is
      essential for specificity, whereas interactions between the SARA beta strand and 
      the Smad2 three-helix bundle contribute significantly to binding affinity.
      Comparison of the structures between Smad2 and a comediator Smad suggests a model
      for how receptor-regulated Smads are recognized by the type I receptors.
FAU - Wu, G
AU  - Wu G
AD  - Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory,
      Princeton, NJ 08544, USA.
FAU - Chen, Y G
AU  - Chen YG
FAU - Ozdamar, B
AU  - Ozdamar B
FAU - Gyuricza, C A
AU  - Gyuricza CA
FAU - Chong, P A
AU  - Chong PA
FAU - Wrana, J L
AU  - Wrana JL
FAU - Massague, J
AU  - Massague J
FAU - Shi, Y
AU  - Shi Y
LA  - eng
SI  - PDB/1DEV
GR  - CA85171/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Smad2 Protein)
RN  - 0 (Trans-Activators)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.30 (Activin Receptors, Type I)
RN  - EC 2.7.11.30 (Receptor, Transforming Growth Factor-beta Type I)
SB  - IM
MH  - *Activin Receptors, Type I
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Carrier Proteins/*chemistry/*metabolism
MH  - Crystallography, X-Ray
MH  - DNA-Binding Proteins/*chemistry/genetics/*metabolism
MH  - Hydrogen Bonding
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phosphorylation
MH  - Point Mutation
MH  - Protein Conformation
MH  - Protein Serine-Threonine Kinases/chemistry/genetics/metabolism
MH  - Protein Structure, Secondary
MH  - Protein Structure, Tertiary
MH  - Receptor, Transforming Growth Factor-beta Type I
MH  - Receptors, Transforming Growth Factor beta/chemistry/genetics/metabolism
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Signal Transduction
MH  - Smad2 Protein
MH  - Trans-Activators/*chemistry/genetics/*metabolism
MH  - Zinc Fingers
EDAT- 1999/12/30 00:00
MHDA- 1999/12/30 00:01
CRDT- 1999/12/30 00:00
PHST- 1999/12/30 00:00 [pubmed]
PHST- 1999/12/30 00:01 [medline]
PHST- 1999/12/30 00:00 [entrez]
AID - 8162 [pii]
AID - 10.1126/science.287.5450.92 [doi]
PST - ppublish
SO  - Science. 2000 Jan 7;287(5450):92-7. doi: 10.1126/science.287.5450.92.