PMID- 10614782
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 66
IP  - 6
DP  - 1999 Dec
TI  - The individual regulation of granule protein mRNA levels during neutrophil
      maturation explains the heterogeneity of neutrophil granules.
PG  - 989-95
AB  - The in vivo mRNA levels for 16 granule proteins during neutrophil differentiation
      were determined to address the question of whether the synthesis of granule
      proteins is regulated individually or blockwise. RNA was extracted from
      peripheral blood granulocytes and three different populations of neutrophil
      precursors isolated from human bone marrow by Percoll density centrifugation. The
      mRNA levels in relation to the maturation of the cells were determined by
      Northern blot for the 12 matrix proteins myeloperoxidase, proteinase-3, elastase,
      defensin, lactoferrin, NGAL, hCAP-18, transcobalamin-I, SGP28, gelatinase,
      lysozyme, and serglycin and the 4 membrane proteins CD68, CD11b,
      N-formyl-methionyl-leucyl-phenylalanine receptor, and CD35. This panel of
      transcripts ensured that markers for all exocytosable organelles of the
      neutrophil were included in the study. A highly differentiated distribution of
      mRNAs for granule proteins was demonstrated that can explain the heterogeneity of
      the intracellular storage granules and secretory vesicles of the neutrophil.
      Furthermore, the individual distribution of these transcripts provides the basis 
      for a more detailed assessment of neutrophil maturation than that obtained by
      morphological studies or the use of a single marker protein for azurophil,
      specific, and gelatinase granules.
FAU - Cowland, J B
AU  - Cowland JB
AD  - The Finsen Centre, Department of Hematology, Rigshospitalet, University of
      Copenhagen, Denmark. jcowland@rh.dk
FAU - Borregaard, N
AU  - Borregaard N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Blood Proteins)
RN  - 0 (Defensins)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.4.21.- (Lactoferrin)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
RN  - EC 3.4.21.76 (Myeloblastin)
RN  - EC 3.4.24.- (Gelatinases)
SB  - IM
CIN - J Leukoc Biol. 1999 Dec;66(6):867-8. PMID: 10614766
MH  - Blood Proteins/biosynthesis
MH  - Blotting, Northern
MH  - Bone Marrow Cells/cytology/enzymology/metabolism
MH  - Cell Differentiation/physiology
MH  - Cellular Senescence/physiology
MH  - Cytoplasmic Granules/classification/enzymology/*metabolism
MH  - Defensins
MH  - Extracellular Matrix Proteins/*biosynthesis/blood
MH  - Gelatinases/biosynthesis/blood
MH  - Granulocytes/cytology/enzymology/metabolism
MH  - Humans
MH  - Lactoferrin/biosynthesis/blood
MH  - Leukocyte Elastase/biosynthesis/blood
MH  - Muramidase/biosynthesis/blood
MH  - Myeloblastin
MH  - Neutrophils/*cytology/*metabolism/ultrastructure
MH  - Peroxidase/biosynthesis/blood
MH  - Protein Biosynthesis
MH  - RNA, Messenger/*metabolism
MH  - Serine Endopeptidases/biosynthesis/blood
EDAT- 1999/12/30 00:00
MHDA- 1999/12/30 00:01
CRDT- 1999/12/30 00:00
PHST- 1999/12/30 00:00 [pubmed]
PHST- 1999/12/30 00:01 [medline]
PHST- 1999/12/30 00:00 [entrez]
AID - 10.1002/jlb.66.6.989 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1999 Dec;66(6):989-95. doi: 10.1002/jlb.66.6.989.