PMID- 10614670
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20061115
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 141
IP  - 1
DP  - 2000 Jan
TI  - A central domain binding site in insulin-like growth factor binding protein-5 for
      the acid-labile subunit.
PG  - 454-7
AB  - Like insulin-like growth factor binding protein-3 (IGFBP-3), IGFBP-5 forms a
      ternary complex with insulin-like growth factor (IGF)-I or IGF-II, and the
      acid-labile subunit (ALS). The study of IGFBP-5/IGFBP-6 chimeric proteins with
      amino-terminal and middle domain swaps, has revealed the existence of a site in
      the middle domain of IGFBP-5, that binds to ALS in the absence of the IGFBP-5
      carboxy-terminal domain. An IGFBP-6 chimeric protein containing the central
      domain of IGFBP-5 complexed efficiently with ALS, and a carboxy-terminally
      truncated IGFBP-5 mutant, IGFBP-5'(1-169), also bound to ALS in the presence of
      IGFs, although with much less potency than full length rhIGFBP-5. In contrast to 
      the latter, IGFBP-5(1-169) preferentially formed ternary complexes with IGF-II
      rather than IGF-I. These results indicate that a site which binds ALS exists in
      IGFBP-5 mutants which lack the IGFBP-5 carboxy-terminal domain.
FAU - Twigg, S M
AU  - Twigg SM
AD  - Kolling Institute of Medical Research, University of Sydney, Royal North Shore
      Hospital, St Leonards, NSW, Australia.
FAU - Kiefer, M C
AU  - Kiefer MC
FAU - Zapf, J
AU  - Zapf J
FAU - Baxter, R C
AU  - Baxter RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Carrier Proteins)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Glycoproteins)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 5)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Somatomedins)
RN  - 0 (insulin-like growth factor binding protein, acid labile subunit)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
SB  - AIM
SB  - IM
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Carrier Proteins/*chemistry/genetics
MH  - Cross-Linking Reagents
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glycoproteins/*chemistry/genetics
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Protein 5/*chemistry/genetics
MH  - Insulin-Like Growth Factor Binding Protein 6/chemistry/genetics
MH  - Insulin-Like Growth Factor I/chemistry
MH  - Insulin-Like Growth Factor II/chemistry
MH  - Precipitin Tests
MH  - Recombinant Proteins/chemistry
MH  - Silver Staining
MH  - Somatomedins/*chemistry/genetics
EDAT- 1999/12/30 00:00
MHDA- 1999/12/30 00:01
CRDT- 1999/12/30 00:00
PHST- 1999/12/30 00:00 [pubmed]
PHST- 1999/12/30 00:01 [medline]
PHST- 1999/12/30 00:00 [entrez]
AID - 10.1210/endo.141.1.7375 [doi]
PST - ppublish
SO  - Endocrinology. 2000 Jan;141(1):454-7. doi: 10.1210/endo.141.1.7375.