PMID- 10613913
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20190703
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 147
IP  - 7
DP  - 1999 Dec 27
TI  - Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2,
      ZO-3, and myosin.
PG  - 1569-82
AB  - We characterized the sequence and protein interactions of cingulin, an M(r)
      140-160-kD phosphoprotein localized on the cytoplasmic surface of epithelial
      tight junctions (TJ). The derived amino acid sequence of a full-length Xenopus
      laevis cingulin cDNA shows globular head (residues 1-439) and tail (1,326-1,368) 
      domains and a central alpha-helical rod domain (440-1,325). Sequence analysis,
      electron microscopy, and pull-down assays indicate that the cingulin rod is
      responsible for the formation of coiled-coil parallel dimers, which can further
      aggregate through intermolecular interactions. Pull-down assays from epithelial, 
      insect cell, and reticulocyte lysates show that an NH(2)-terminal fragment of
      cingulin (1-378) interacts in vitro with ZO-1 (K(d) approximately 5 nM), ZO-2,
      ZO-3, myosin, and AF-6, but not with symplekin, and a COOH-terminal fragment
      (377-1,368) interacts with myosin and ZO-3. ZO-1 and ZO-2 immunoprecipitates
      contain cingulin, suggesting in vivo interactions. Full-length cingulin, but not 
      NH(2)-terminal and COOH-terminal fragments, colocalizes with endogenous cingulin 
      in transfected MDCK cells, indicating that sequences within both head and rod
      domains are required for TJ localization. We propose that cingulin is a
      functionally important component of TJ, linking the submembrane plaque domain of 
      TJ to the actomyosin cytoskeleton.
FAU - Cordenonsi, M
AU  - Cordenonsi M
AD  - Department of Biology, University of Padova, 35121 Padova, Italy.
FAU - D'Atri, F
AU  - D'Atri F
FAU - Hammar, E
AU  - Hammar E
FAU - Parry, D A
AU  - Parry DA
FAU - Kendrick-Jones, J
AU  - Kendrick-Jones J
FAU - Shore, D
AU  - Shore D
FAU - Citi, S
AU  - Citi S
LA  - eng
SI  - GENBANK/AF207901
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (CGN protein, Xenopus)
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphoproteins)
RN  - 0 (TJP1 protein, Xenopus)
RN  - 0 (Xenopus Proteins)
RN  - 0 (Zonula Occludens Proteins)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 0 (Zonula Occludens-2 Protein)
RN  - EC 3.6.4.1 (Myosins)
RN  - EC 3.6.4.4 (Kinesin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/*metabolism
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Chickens
MH  - Cytoplasm/metabolism
MH  - Dogs
MH  - Kinesin/metabolism
MH  - Membrane Proteins/*chemistry/*metabolism/physiology
MH  - Molecular Sequence Data
MH  - Myosins/*metabolism
MH  - Peptide Fragments/metabolism
MH  - Phosphoproteins/*metabolism
MH  - Protein Structure, Tertiary
MH  - Sequence Analysis, Protein
MH  - Transfection
MH  - *Xenopus Proteins
MH  - Xenopus laevis
MH  - Zonula Occludens Proteins
MH  - Zonula Occludens-1 Protein
MH  - Zonula Occludens-2 Protein
PMC - PMC2174252
EDAT- 1999/12/30 00:00
MHDA- 1999/12/30 00:01
CRDT- 1999/12/30 00:00
PHST- 1999/12/30 00:00 [pubmed]
PHST- 1999/12/30 00:01 [medline]
PHST- 1999/12/30 00:00 [entrez]
AID - 10.1083/jcb.147.7.1569 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Dec 27;147(7):1569-82. doi: 10.1083/jcb.147.7.1569.