PMID- 10612803
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20220129
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 27
IP  - 2
DP  - 2000 Feb
TI  - Narrowing of the region of allelic loss in 21q11-21 in squamous non-small cell
      lung carcinoma and cloning of a novel ubiquitin-specific protease gene from the
      deleted segment.
PG  - 153-61
AB  - We examined 42 fresh non-small cell lung carcinomas for allelic loss using 4
      microsatellite markers located in a 4.5 Mb region in 21q11-21, a gene-poor
      interval recently found by others to be homozygously deleted and exhibiting
      frequent allelic loss in lung cancer. We found allelic loss across the entire
      segment in 13/34 informative squamous carcinomas, with 2 cases showing loss in
      only part of the region. Analysis by fluorescence in situ hybridization of
      P1-derived artificial chromosomes from the region directly on paraffin sections
      of the tumor is in concordance with the loss of heterozygosity (LOH) results, and
      tentatively excludes a 2 Mb segment bearing 2 of the only 3 known genes in the
      area. Exon trapping in the remaining segment of loss led to identification and
      cloning of a novel gene spanning 150 kb within the deletion. The full-length gene
      encodes a protein of 1,055 amino acids with homology to ubiquitin-specific
      proteases across the eukaryotic evolutionary spectrum. The expressed protein acts
      as a de-ubiquitinating enzyme as proved by the ability to cleave ubiquitin from a
      model fusion protein. We found no mutations in the sequence of the functional
      domains of this gene in any of the LOH-exhibiting tumor DNA samples. It is,
      however, interesting that genes of the same superfamily have been reported on
      3p21, a locus showing the most frequent allelic instability and deletions in lung
      cancer. Genes Chromosomes Cancer 27:153-161, 2000.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Groet, J
AU  - Groet J
AD  - Centre for Applied Molecular Biology, School of Pharmacy, University of London,
      London, United Kingdom.
FAU - Ives, J H
AU  - Ives JH
FAU - Jones, T A
AU  - Jones TA
FAU - Danton, M
AU  - Danton M
FAU - Flomen, R H
AU  - Flomen RH
FAU - Sheer, D
AU  - Sheer D
FAU - Hrascan, R
AU  - Hrascan R
FAU - Pavelic, K
AU  - Pavelic K
FAU - Nizetic, D
AU  - Nizetic D
LA  - eng
SI  - GENBANK/AF134213
GR  - A3585/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ubiquitins)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.19.12 (Ubiquitin-Specific Proteases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carcinoma, Non-Small-Cell Lung/*genetics
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Cloning, Molecular
MH  - DNA/chemistry/genetics
MH  - Endopeptidases/*genetics/metabolism
MH  - Exons
MH  - Genetic Vectors
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - Lung Neoplasms/*genetics
MH  - Microsatellite Repeats
MH  - Molecular Sequence Data
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Ubiquitin-Specific Proteases
MH  - Ubiquitins/genetics/metabolism
EDAT- 1999/12/29 00:00
MHDA- 1999/12/29 00:01
CRDT- 1999/12/29 00:00
PHST- 1999/12/29 00:00 [pubmed]
PHST- 1999/12/29 00:01 [medline]
PHST- 1999/12/29 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200002)27:2<153::AID-GCC6>3.0.CO;2-A [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Feb;27(2):153-61.