PMID- 10612437
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 156
IP  - 1-2
DP  - 1999 Oct 25
TI  - Localization of growth differentiation factor-9 (GDF-9) mRNA and protein in rat
      ovaries and cDNA cloning of rat GDF-9 and its novel homolog GDF-9B.
PG  - 189-93
AB  - Although targeted gene disruption of GDF-9, an oocyte derived growth factor,
      leads to an arrest of folliculogenesis and causes infertility in female mice,
      little is known on the expression of GDF-9 protein in the ovary. We show that
      GDF-9 protein is expressed in rat oocytes during folliculogenesis from the early 
      primary follicle stage onwards but the most intensive immunostaining was seen in 
      primary and preantral follicles. Northern blot analyses of the ontogeny of GDF-9 
      gene expression in postnatal rat ovaries showed that the GDF-9 transcript levels 
      are clearly increased on the second postnatal day concomitant with the appearance
      of primary follicles. Interestingly, Northern blot and in situ hybridization
      analyses indicate a similar expression pattern for GDF-9B, the rat ortholog of a 
      mouse GDF-9 like factor for which we recently reported the partial amino acid
      sequence. The polypeptide sequences deduced from isolated ovarian cDNAs indicate 
      that the rat GDF-9 prepropeptide is 440 amino acids (aa) in length and the
      putative mature peptide is 135 aa whereas rat GDF-9B is 391 aa long and the
      mature region is 125 aa. We conclude that (1) the GDF-9 protein is highly
      expressed in the oocytes of primary follicles of rat ovaries suggesting that it
      plays a role mainly in early folliculogenesis and that (2) the full-length
      polypeptide sequence of GDF-9B suggests that this novel TGF-beta family member is
      likely to be a secreted growth factor that may regulate folliculogenesis at
      similar developmental stages as GDF-9.
FAU - Jaatinen, R
AU  - Jaatinen R
AD  - Department of Bacteriology and Immunology, Haartman Institute, University of
      Helsinki, Finland.
FAU - Laitinen, M P
AU  - Laitinen MP
FAU - Vuojolainen, K
AU  - Vuojolainen K
FAU - Aaltonen, J
AU  - Aaltonen J
FAU - Louhio, H
AU  - Louhio H
FAU - Heikinheimo, K
AU  - Heikinheimo K
FAU - Lehtonen, E
AU  - Lehtonen E
FAU - Ritvos, O
AU  - Ritvos O
LA  - eng
SI  - GENBANK/AJ132406
SI  - GENBANK/AJ132407
SI  - GENBANK/X81899
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Bmp15 protein, mouse)
RN  - 0 (Bmp15 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 15)
RN  - 0 (DNA, Complementary)
RN  - 0 (Gdf9 protein, mouse)
RN  - 0 (Gdf9 protein, rat)
RN  - 0 (Growth Differentiation Factor 9)
RN  - 0 (Growth Substances)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Aging
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Bone Morphogenetic Protein 15
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Growth Differentiation Factor 9
MH  - Growth Substances/chemistry/*genetics
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oocytes/metabolism
MH  - Ovarian Follicle/metabolism
MH  - Ovary/*metabolism
MH  - Protein Sorting Signals/genetics
MH  - RNA, Messenger/analysis/*genetics
MH  - Rats
MH  - Transcription, Genetic
MH  - Transforming Growth Factor beta/genetics
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - S0303-7207(99)00100-8 [pii]
AID - 10.1016/s0303-7207(99)00100-8 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1999 Oct 25;156(1-2):189-93. doi:
      10.1016/s0303-7207(99)00100-8.