PMID- 10612428
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 156
IP  - 1-2
DP  - 1999 Oct 25
TI  - Melatonin receptor mRNA expression in human granulosa cells.
PG  - 107-10
AB  - We have shown that the melatonin receptor agonist, 2-[125I] iodomelatonin, binds 
      to high-affinity guanine nucleotide-sensitive sites on human granulosa (HG) cell 
      membranes. In order to confirm the presence of melatonin receptors in HG cells,
      we have now used a reverse transcriptase-polymerase chain reaction (RT-PCR)
      procedure to examine receptor subtype expression. RT-PCR studies revealed the
      presence of the mt1 (Mel1alpha) melatonin receptor subtype in ten single or
      pooled HG cell samples which were obtained from 14 patients. In contrast,
      expression of MT2 ( Mel1b) mRNA was observed in only two of these HG samples. DNA
      sequencing of the mt1 PCR product confirmed its identity with the reported human 
      mt1 melatonin receptor. The expression of mt1 and MT2 receptor mRNA in HG cells
      and the reported presence of melatonin in human follicular fluid indicate a
      potentially important role for this hormone in regulating human ovarian and
      reproductive function.
FAU - Niles, L P
AU  - Niles LP
AD  - Department of Biomedical Sciences, McMaster University, Faculty of Health
      Sciences, Hamilton, Ontario, Canada. niles@fhs.csu.mcmaster.ca
FAU - Wang, J
AU  - Wang J
FAU - Shen, L
AU  - Shen L
FAU - Lobb, D K
AU  - Lobb DK
FAU - Younglai, E V
AU  - Younglai EV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Melatonin)
RN  - 93515-00-5 (2-iodomelatonin)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Granulosa Cells/*metabolism
MH  - Humans
MH  - Iodine Radioisotopes
MH  - Melatonin/analogs & derivatives/pharmacokinetics
MH  - RNA, Messenger/*genetics
MH  - Receptors, Cell Surface/*genetics/metabolism
MH  - Receptors, Cytoplasmic and Nuclear/*genetics/metabolism
MH  - Receptors, Melatonin
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seasons
MH  - *Transcription, Genetic
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - S0303-7207(99)00135-5 [pii]
AID - 10.1016/s0303-7207(99)00135-5 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1999 Oct 25;156(1-2):107-10. doi:
      10.1016/s0303-7207(99)00135-5.