PMID- 10612400
OWN - NLM
STAT- MEDLINE
DCOM- 20000118
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 99
IP  - 6
DP  - 1999 Dec 10
TI  - CNS myelin and sertoli cell tight junction strands are absent in Osp/claudin-11
      null mice.
PG  - 649-59
AB  - Oligodendrocyte-specific protein (OSP)/claudin-11 is a recently identified
      transmembrane protein found in CNS myelin and testis with unknown function.
      Herein we demonstrate that Osp null mice exhibit both neurological and
      reproductive deficits: CNS nerve conduction is slowed, hindlimb weakness is
      conspicuous, and males are sterile. Freeze fracture reveals that tight junction
      intramembranous strands are absent in CNS myelin and between Sertoli cells of
      mutant mice. Our results demonstrate that OSP is the mediator of parallel-array
      tight junction strands and distinguishes this protein from other intrinsic
      membrane proteins in tight junctions. These novel results provide direct evidence
      of the pivotal role of the claudin family in generating the paracellular physical
      barrier of tight junctions necessary for spermatogenesis and normal CNS function.
FAU - Gow, A
AU  - Gow A
AD  - Brookdale Center for Developmental and Molecular Biology, Mount Sinai School of
      Medicine, New York, New York 10029, USA.
FAU - Southwood, C M
AU  - Southwood CM
FAU - Li, J S
AU  - Li JS
FAU - Pariali, M
AU  - Pariali M
FAU - Riordan, G P
AU  - Riordan GP
FAU - Brodie, S E
AU  - Brodie SE
FAU - Danias, J
AU  - Danias J
FAU - Bronstein, J M
AU  - Bronstein JM
FAU - Kachar, B
AU  - Kachar B
FAU - Lazzarini, R A
AU  - Lazzarini RA
LA  - eng
GR  - 1K08 EY 00390-01A1/EY/NEI NIH HHS/United States
GR  - NS01596/NS/NINDS NIH HHS/United States
GR  - NS33165/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Claudins)
RN  - 0 (Cldn11 protein, mouse)
RN  - 0 (Cldn11 protein, rat)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Brain/cytology/*metabolism
MH  - Claudins
MH  - Freeze Fracturing
MH  - Gene Expression Regulation, Developmental
MH  - Haplorhini
MH  - Hindlimb/growth & development
MH  - Infertility, Male/metabolism/pathology
MH  - Male
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Confocal
MH  - Microscopy, Electron
MH  - Myelin Sheath/*metabolism
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Oligodendroglia/cytology/metabolism
MH  - Rats
MH  - Sertoli Cells/*metabolism/ultrastructure
MH  - Testis/pathology
MH  - Tight Junctions/*metabolism/ultrastructure
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - S0092-8674(00)81553-6 [pii]
AID - 10.1016/s0092-8674(00)81553-6 [doi]
PST - ppublish
SO  - Cell. 1999 Dec 10;99(6):649-59. doi: 10.1016/s0092-8674(00)81553-6.