PMID- 10612039
OWN - NLM
STAT- MEDLINE
DCOM- 20000131
LR  - 20191210
IS  - 0965-1748 (Print)
IS  - 0965-1748 (Linking)
VI  - 29
IP  - 12
DP  - 1999 Dec
TI  - Drosophila seminal fluid proteins enter the circulatory system of the mated 
      female fly by crossing the posterior vaginal wall.
PG  - 1043-52
AB  - Seminal fluid proteins from males of many insect species affect the behavior and 
      physiology of their mates. In some cases, these effects result from entry of the 
      proteins into the female's circulatory system. In the fruit fly Drosophila 
      melanogaster, some seminal fluid proteins enter the female's circulatory system 
      after transfer from the male while others remain confined within the reproductive 
      tract. To address where and how seminal fluid proteins enter the hemolymph of the 
      mated female, we compared the kinetics of transfer and localization in mated 
      females of two seminal fluid proteins that enter the hemolymph (Acp26Aa and 
      Acp62F) and one that does not (Acp36DE). We also generated transgenic flies that 
      produce Acp26Aa tagged with Aequorea victoria green fluorescent protein (GFP) to 
      monitor its transfer in vivo. We report that Acps enter the female circulatory 
      system from the posterior vagina immediately after insemination. The ability of 
      Acps to enter the female hemolymph correlates with their ability to cross the 
      intima that lines the posterior vagina. The ventral posterior vagina is 
      structurally unlike other parts of the female reproductive tract in that it lacks 
      muscles. We hypothesize that it has higher permeability thus affording access to 
      the female's circulatory system.
FAU - Lung, O
AU  - Lung O
AD  - Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 
      14853, USA.
FAU - Wolfner, M F
AU  - Wolfner MF
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Acp26Aa protein, Drosophila)
RN  - 0 (Acp36DE protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Peptides)
RN  - 0 (SP protein, Drosophila)
RN  - 0 (male accessory gland peptide, Drosophila)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - *Drosophila Proteins
MH  - Drosophila melanogaster/*physiology
MH  - Female
MH  - Hemolymph/metabolism
MH  - Intercellular Signaling Peptides and Proteins
MH  - Male
MH  - Peptides/genetics/*physiology
MH  - Semen/*physiology
MH  - *Sexual Behavior, Animal
MH  - Time Factors
MH  - Vagina
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - S0965174899000788 [pii]
AID - 10.1016/s0965-1748(99)00078-8 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 1999 Dec;29(12):1043-52. doi: 
      10.1016/s0965-1748(99)00078-8.