PMID- 10611498
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 118
IP  - 1-2
DP  - 1999 Dec 10
TI  - Developmental expression of palmitoyl protein thioesterase in normal mice.
PG  - 1-11
AB  - Deficiency in palmitoyl protein thioesterase (PPT) results in the rapid death of 
      neocortical neurons in human. Very little is known about the developmental and
      cell-specific expression of this lysosomal enzyme. Here we show that PPT is
      expressed as a major 2.65 kb and a minor 1.85 kb transcript in the mouse brain.
      Transcript levels gradually increase between postnatal days 10 and 30. In situ
      hybridization analysis revealed that PPT transcripts are found widely but not
      homogeneously in the brain. The most intense signal was detected in the cerebral 
      cortex (layers II, IV-V), hippocampal CA1-CA3 pyramidal cells, dentate gyrus
      granule cells and the hypothalamus. Immunostaining of PPT was localized in the
      cell soma, axons and dendrites, especially in the pyramidal and granular cells of
      the hippocampus, correlating well, both spatially and temporally, with the
      immunoreactivity of a presynaptic vesicle membrane protein, synaptophysin. In
      whole embryos, at embryonic day 8, the PPT mRNA expression was most apparent
      throughout the neuroepithelium, and from day 9 onwards it was seen in all
      tissues. The expression pattern of PPT suggests its general significance for the 
      brain cells and reflects the response to maturation and growth of the neural
      networks. Strong PPT immunoreactivity in the axons and dentrites would imply that
      PPT may not be exclusively a lysosomal enzyme. A notable correlation with
      synaptophysin would suggest that PPT may have a role in the function of the
      synaptic machinery.
FAU - Isosomppi, J
AU  - Isosomppi J
AD  - Department of Human Molecular Genetics, National Public Health Institute and
      Department of Medical Genetics, University of Helsinki, Mannerheimintie 166,
      FIN-00300, Helsinki, Finland.
FAU - Heinonen, O
AU  - Heinonen O
FAU - Hiltunen, J O
AU  - Hiltunen JO
FAU - Greene, N D
AU  - Greene ND
FAU - Vesa, J
AU  - Vesa J
FAU - Uusitalo, A
AU  - Uusitalo A
FAU - Mitchison, H M
AU  - Mitchison HM
FAU - Saarma, M
AU  - Saarma M
FAU - Jalanko, A
AU  - Jalanko A
FAU - Peltonen, L
AU  - Peltonen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.2.- (Thiolester Hydrolases)
RN  - EC 3.1.2.22 (palmitoyl-protein thioesterase)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Animals, Newborn/growth & development/*metabolism
MH  - Brain/*embryology/*metabolism
MH  - Embryonic and Fetal Development
MH  - Fetus/*metabolism/physiology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Inbred Strains
MH  - RNA, Messenger/metabolism
MH  - Reference Values
MH  - Thiolester Hydrolases/genetics/*metabolism
MH  - Tissue Distribution
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - S0165380699001157 [pii]
AID - 10.1016/s0165-3806(99)00115-7 [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1999 Dec 10;118(1-2):1-11. doi:
      10.1016/s0165-3806(99)00115-7.