PMID- 10611342
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 26
DP  - 1999 Dec 21
TI  - Constitutive tyrosine phosphorylation of the inhibitory paired Ig-like receptor
      PIR-B.
PG  - 15086-90
AB  - PIR-A and PIR-B are activating and inhibitory Ig-like receptors on murine B
      lymphocytes, dendritic cells, and myeloid-lineage cells. The inhibitory function 
      of PIR-B is mediated via its cytoplasmic immunoreceptor tyrosine-based inhibitory
      motifs, whereas PIR-A pairs with the Fc receptor common gamma chain to form an
      activating receptor complex. In these studies, we observed constitutive tyrosine 
      phosphorylation of PIR-B molecules on macrophages and B lymphocytes, irrespective
      of the cell activation status. Splenocyte PIR-B molecules were constitutively
      associated with the SHP-1 protein tyrosine phosphatase and Lyn protein tyrosine
      kinase. In Lyn-deficient mice, PIR-B tyrosine phosphorylation was greatly
      reduced. Unexpectedly, tyrosine phosphorylation of PIR-B was not observed in most
      myeloid and B cell lines but could be induced by ligation of the PIR molecules.
      Finally, the phosphorylation status of PIR-B was significantly reduced in MHC
      class I-deficient mice, although not in mice deficient in TAP1 or MHC class II
      expression. These findings suggest a physiological inhibitory role for PIR-B that
      is regulated by endogenous MHC class I-like ligands.
FAU - Ho, L H
AU  - Ho LH
AD  - Division of Developmental Immunology, University of Alabama at Birmingham,
      Birmingham, AL 35294-3300, USA.
FAU - Uehara, T
AU  - Uehara T
FAU - Chen, C C
AU  - Chen CC
FAU - Kubagawa, H
AU  - Kubagawa H
FAU - Cooper, M D
AU  - Cooper MD
LA  - eng
GR  - AI42127/AI/NIAID NIH HHS/United States
GR  - R01 AI039816/AI/NIAID NIH HHS/United States
GR  - R37 AI039816/AI/NIAID NIH HHS/United States
GR  - R01 AI042127/AI/NIAID NIH HHS/United States
GR  - AI39816/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Pirb protein, mouse)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (TAP1 protein, human)
RN  - 0 (Tap1 protein, mouse)
RN  - 42HK56048U (Tyrosine)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters
MH  - Animals
MH  - B-Lymphocytes/cytology/immunology
MH  - Bone Marrow Cells/cytology
MH  - Cell Lineage
MH  - Histocompatibility Antigens Class I
MH  - Histocompatibility Antigens Class II
MH  - Lymphocyte Activation
MH  - Major Histocompatibility Complex
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mice, Mutant Strains
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Processing, Post-Translational
MH  - Receptors, Immunologic/*metabolism
MH  - Tyrosine/*metabolism
PMC - PMC24777
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - 10.1073/pnas.96.26.15086 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15086-90. doi:
      10.1073/pnas.96.26.15086.