PMID- 10611263
OWN - NLM
STAT- MEDLINE
DCOM- 20000229
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 6
IP  - 1
DP  - 2000 Jan
TI  - Identification of carbonic anhydrase XII as the membrane isozyme expressed in the
      normal human endometrial epithelium.
PG  - 68-74
AB  - Although previous studies demonstrated carbonic anhydrase (CA) activity in the
      human endometrium, the CA isozyme(s) responsible for this activity has not been
      established. In this report, we provide the first evidence that the CA isozyme
      XII, a recently identified transmembrane isozyme that is expressed in normal
      kidney and greatly overexpressed in some renal cancers, is present in
      endometrium. We show by immunohistochemistry that CA XII is expressed in the
      basolateral plasma membrane of epithelial cells of normal human endometrium.
      Expression of CA XII in uterus was confirmed by Northern blotting.
      Detergent-solubilized CA XII was isolated from human endometrium by inhibitor
      affinity chromatography and characterized by isoelectric focusing and Western
      blot as a polypeptide with a pI of 6.3. The high expression of CA XII in the
      endometrial epithelium suggests that it may be functionally linked to the
      pH-dependent events in spermatozoa that precede fertilization. Its basolateral
      location and extracellular active site could also allow it to influence the
      morphological changes in endometrium that occur during the menstrual cycle.
FAU - Karhumaa, P
AU  - Karhumaa P
AD  - Department of Anatomy and Cell Biology, University of Oulu, Box 5000, FIN-90401
      Oulu, Finland. of Saarland, 66421 Homburg, Germany.
FAU - Parkkila, S
AU  - Parkkila S
FAU - Tureci, O
AU  - Tureci O
FAU - Waheed, A
AU  - Waheed A
FAU - Grubb, J H
AU  - Grubb JH
FAU - Shah, G
AU  - Shah G
FAU - Parkkila, A
AU  - Parkkila A
FAU - Kaunisto, K
AU  - Kaunisto K
FAU - Tapanainen, J
AU  - Tapanainen J
FAU - Sly, W S
AU  - Sly WS
FAU - Rajaniemi, H
AU  - Rajaniemi H
LA  - eng
GR  - DK 40163/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Isoenzymes)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - CHO Cells
MH  - Carbonic Anhydrases/*biosynthesis/genetics/immunology/isolation & purification
MH  - Cell Membrane/metabolism
MH  - Cricetinae
MH  - Endometrium/*enzymology/pathology
MH  - Epithelium/enzymology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Isoelectric Focusing
MH  - Isoenzymes/*biosynthesis/genetics/immunology/isolation & purification
EDAT- 1999/12/28 09:00
MHDA- 2000/03/04 09:00
CRDT- 1999/12/28 09:00
PHST- 1999/12/28 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/12/28 09:00 [entrez]
AID - 10.1093/molehr/6.1.68 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2000 Jan;6(1):68-74. doi: 10.1093/molehr/6.1.68.