PMID- 10610771
OWN - NLM
STAT- MEDLINE
DCOM- 20000118
LR  - 20171116
IS  - 0022-2836 (Print)
IS  - 0022-2836 (Linking)
VI  - 294
IP  - 2
DP  - 1999 Nov 26
TI  - Dominance of intrinsic genetic factors in shaping the human immunoglobulin
      Vlambda repertoire.
PG  - 457-65
AB  - The expressed human immunoglobulin Vlambda repertoire demonstrates a strong bias 
      in the use of individual Vlambda segments. Mechanisms that underlie such biases
      can be divided into two categories: intrinsic genetic processes that lead to the 
      preferential rearrangement and/or expression of certain segments; and selection
      following light chain expression. Here, we have used two approaches to
      investigate the factors that shape the human Vlambda repertoire. Firstly, we
      characterised 136 Vlambda rearrangements (59 productive and 77 non-productive)
      amplified from the human genomic DNA of peripheral blood cells. Secondly, we
      analysed Vlambda segment use in a library of 2000 cDNA clones from a transgenic
      mouse containing a 380 kb region (including 15 functional Vlambda segments) from 
      the human immunoglobulin lambda locus. By hybridisation and sequencing we found
      that the patterns of use of human Vlambda segments in the transgenic mouse were
      similar to those found in the expressed human peripheral blood repertoire and in 
      productive and non-productive genomic DNA rearrangements. These data indicate the
      importance of intrinsic genetic factors in shaping the human Vlambda repertoire
      and highlight the remarkable conservation of the molecular mechanisms involved in
      the production of the antibody repertoire in mouse and man. Therefore, transgenic
      mice represent a good model for analysis of the human antibody repertoire and for
      the production of human antibodies.
CI  - Copyright 1999 Academic Press.
FAU - Ignatovich, O
AU  - Ignatovich O
AD  - MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK.
      oil@mrc-lmb.cam.ac.uk
FAU - Tomlinson, I M
AU  - Tomlinson IM
FAU - Popov, A V
AU  - Popov AV
FAU - Bruggemann, M
AU  - Bruggemann M
FAU - Winter, G
AU  - Winter G
LA  - eng
SI  - GENBANK/AF194540
SI  - GENBANK/AF194541
SI  - GENBANK/AF194542
SI  - GENBANK/AF194543
SI  - GENBANK/AF194544
SI  - GENBANK/AF194545
SI  - GENBANK/AF194546
SI  - GENBANK/AF194547
SI  - GENBANK/AF194548
SI  - GENBANK/AF194549
SI  - GENBANK/AF194550
SI  - GENBANK/AF194551
SI  - GENBANK/AF194552
SI  - GENBANK/AF194553
SI  - GENBANK/AF194554
SI  - GENBANK/AF194555
SI  - GENBANK/AF194556
SI  - GENBANK/AF194557
SI  - GENBANK/AF194558
SI  - GENBANK/AF194559
SI  - GENBANK/AF194560
SI  - GENBANK/AF194561
SI  - GENBANK/AF194562
SI  - GENBANK/AF194563
SI  - GENBANK/AF194564
SI  - GENBANK/AF194565
SI  - GENBANK/AF194566
SI  - GENBANK/AF194567
SI  - GENBANK/AF194568
SI  - GENBANK/AF194569
SI  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Biol
JT  - Journal of molecular biology
JID - 2985088R
RN  - 0 (CD3 Complex)
RN  - 0 (DNA Primers)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 0 (Immunoglobulin kappa-Chains)
RN  - 0 (Immunoglobulin lambda-Chains)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/immunology
MH  - CD3 Complex/genetics
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Gene Rearrangement
MH  - *Genetic Variation
MH  - Humans
MH  - Immunoglobulin Variable Region/*genetics
MH  - Immunoglobulin kappa-Chains/genetics
MH  - Immunoglobulin lambda-Chains/*genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Nucleic Acid Amplification Techniques
MH  - Spleen/cytology/immunology
EDAT- 1999/12/28 00:00
MHDA- 1999/12/28 00:01
CRDT- 1999/12/28 00:00
PHST- 1999/12/28 00:00 [pubmed]
PHST- 1999/12/28 00:01 [medline]
PHST- 1999/12/28 00:00 [entrez]
AID - 10.1006/jmbi.1999.3243 [doi]
AID - S0022-2836(99)93243-6 [pii]
PST - ppublish
SO  - J Mol Biol. 1999 Nov 26;294(2):457-65. doi: 10.1006/jmbi.1999.3243.