PMID- 10610183
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20061115
IS  - 1061-4036 (Print)
IS  - 1061-4036 (Linking)
VI  - 23
IP  - 3
DP  - 1999 Nov
TI  - Leukaemia disease genes: large-scale cloning and pathway predictions.
PG  - 348-53
AB  - Retroviral insertional mutagenesis in BXH2 and AKXD recombinant inbred mice
      induces a high incidence of myeloid or B- and T-cell leukaemia and the proviral
      integration sites in the leukaemias provide powerful genetic tags for disease
      gene identification. Some of the disease genes identified by proviral tagging are
      also associated with human disease, validating this approach for human disease
      gene identification. Although many leukaemia disease genes have been identified
      over the years, many more remain to be cloned. Here we describe an inverse PCR
      (IPCR) method for proviral tagging that makes use of automated DNA sequencing and
      the genetic tools provided by the Mouse Genome Project, which increases the
      throughput for disease gene identification. We also use this IPCR method to clone
      and analyse more than 400 proviral integration sites from AKXD and BXH2
      leukaemias and, in the process, identify more than 90 candidate disease genes.
      Some of these genes function in pathways already implicated in leukaemia, whereas
      others are likely to define new disease pathways. Our studies underscore the
      power of the mouse as a tool for gene discovery and functional genomics.
FAU - Li, J
AU  - Li J
AD  - Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer
      Research and Development Center, Frederick, Maryland, USA.
FAU - Shen, H
AU  - Shen H
FAU - Himmel, K L
AU  - Himmel KL
FAU - Dupuy, A J
AU  - Dupuy AJ
FAU - Largaespada, D A
AU  - Largaespada DA
FAU - Nakamura, T
AU  - Nakamura T
FAU - Shaughnessy, J D Jr
AU  - Shaughnessy JD Jr
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Copeland, N G
AU  - Copeland NG
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Genet
JT  - Nature genetics
JID - 9216904
SB  - IM
CIN - Nat Genet. 1999 Nov;23(3):253-4. PMID: 10545932
MH  - Animals
MH  - Chromosomes/genetics
MH  - Cloning, Molecular/*methods
MH  - Expressed Sequence Tags
MH  - Genome
MH  - Humans
MH  - Leukemia, Experimental/*genetics
MH  - Lymphoma/genetics
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Mutagenesis, Insertional
MH  - Oncogenes/*genetics
MH  - Physical Chromosome Mapping
MH  - Polymerase Chain Reaction/methods
MH  - Proviruses/genetics
MH  - Reproducibility of Results
MH  - Retroviridae/genetics
MH  - Sensitivity and Specificity
MH  - Sequence Homology, Nucleic Acid
MH  - Signal Transduction/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/12/28 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/12/28 09:00
PHST- 1999/12/28 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/12/28 09:00 [entrez]
AID - 10.1038/15531 [doi]
PST - ppublish
SO  - Nat Genet. 1999 Nov;23(3):348-53. doi: 10.1038/15531.