PMID- 10608844
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 53
DP  - 1999 Dec 31
TI  - RA-GEF, a novel Rap1A guanine nucleotide exchange factor containing a
      Ras/Rap1A-associating domain, is conserved between nematode and humans.
PG  - 37815-20
AB  - A yeast two-hybrid screening for Ras-binding proteins in nematode Caenorhabditis 
      elegans has identified a guanine nucleotide exchange factor (GEF) containing a
      Ras/Rap1A-associating (RA) domain, termed Ce-RA-GEF. Both Ce-RA-GEF and its human
      counterpart Hs-RA-GEF possessed a PSD-95/DlgA/ZO-1 (PDZ) domain and a Ras
      exchanger motif (REM) domain in addition to the RA and GEF domains. They also
      contained a region homologous to a cyclic nucleotide monophosphate-binding
      domain, which turned out to be incapable of binding cAMP or cGMP. Although the
      REM and GEF domains are conserved with other GEFs acting on Ras family small
      GTP-binding proteins, the RA and PDZ domains are unseen in any of them. Hs-RA-GEF
      exhibited not only a GTP-dependent binding activity to Rap1A at its RA domain but
      also an activity to stimulate GDP/GTP exchange of Rap1A both in vitro and in vivo
      at the segment containing its REM and GEF domains. However, it did not exhibit
      any binding or GEF activity toward Ras. On the other hand, Ce-RA-GEF associated
      with and stimulated GDP/GTP exchange of both Ras and Rap1A. These results
      indicate that Ce-RA-GEF and Hs-RA-GEF define a novel class of Rap1A GEF
      molecules, which are conserved through evolution.
FAU - Liao, Y
AU  - Liao Y
AD  - Department of Physiology II, Kobe University School of Medicine, 7-5-1
      Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
FAU - Kariya, K
AU  - Kariya K
FAU - Hu, C D
AU  - Hu CD
FAU - Shibatohge, M
AU  - Shibatohge M
FAU - Goshima, M
AU  - Goshima M
FAU - Okada, T
AU  - Okada T
FAU - Watari, Y
AU  - Watari Y
FAU - Gao, X
AU  - Gao X
FAU - Jin, T G
AU  - Jin TG
FAU - Yamawaki-Kataoka, Y
AU  - Yamawaki-Kataoka Y
FAU - Kataoka, T
AU  - Kataoka T
LA  - eng
SI  - GENBANK/AF170796
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RAPGEF2 protein, human)
RN  - 0 (pxf-1 protein, C elegans)
RN  - EC 3.6.5.2 (rap1 GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Caenorhabditis elegans/*chemistry
MH  - *Caenorhabditis elegans Proteins
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Guanine Nucleotide Exchange Factors/*chemistry/genetics/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Nerve Tissue Proteins
MH  - Protein Binding
MH  - Sequence Homology, Amino Acid
MH  - Spodoptera
MH  - rap1 GTP-Binding Proteins/*chemistry/metabolism
MH  - ras Proteins/*chemistry/metabolism
EDAT- 1999/12/23 00:00
MHDA- 1999/12/23 00:01
CRDT- 1999/12/23 00:00
PHST- 1999/12/23 00:00 [pubmed]
PHST- 1999/12/23 00:01 [medline]
PHST- 1999/12/23 00:00 [entrez]
AID - 10.1074/jbc.274.53.37815 [doi]
AID - S0021-9258(19)52964-7 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 31;274(53):37815-20. doi: 10.1074/jbc.274.53.37815.