PMID- 10608812
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 53
DP  - 1999 Dec 31
TI  - Human rabaptin-5 is selectively cleaved by caspase-3 during apoptosis.
PG  - 37583-90
AB  - We have previously shown that Xenopus rabaptin-5 is cleaved in apoptotic
      extracts, with a concomitant reduction in the ability of these extracts to
      support endosomal membrane fusion (Cosulich, S. C., Horiuchi, H., Zerial, M.,
      Clarke, P. R., and Woodman, P. G. (1997) EMBO J. 16, 6182-6191). In this report
      we demonstrate that caspase-dependent cleavage is a conserved feature of
      rabaptin-5. Human rabaptin-5 is cleaved at two sites (HSLD(379) and DESD(438)) in
      apoptotic HeLa extracts. Cleavage is effected by caspase-3, since it is prevented
      when caspase-3 activity is either inhibited by Ac-DEVD-CHO or removed by
      immunodepletion. Moreover, an identical pattern of cleavage is observed using
      recombinant caspase-3. The action of caspase-3 is highly selective; neither
      caspase-2 nor caspase-7 are able to cleave recombinant or cytosolic rabaptin-5.
      Caspase-dependent cleavage of rabaptin-5 generates two physically separated
      coiled coil-forming domains, the C-terminal of which retains the ability to bind 
      the Rab5 exchange factor rabex-5.
FAU - Swanton, E
AU  - Swanton E
AD  - School of Biological Sciences, University of Manchester, Oxford Road, Manchester 
      M13 9PT, United Kingdom.
FAU - Bishop, N
AU  - Bishop N
FAU - Woodman, P
AU  - Woodman P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (Membrane Proteins)
RN  - 0 (RABEP1 protein, human)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Base Sequence
MH  - Caspase 3
MH  - Caspases/*metabolism
MH  - DNA Primers
MH  - Humans
MH  - Hydrolysis
MH  - Membrane Proteins/chemistry/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - *Vesicular Transport Proteins
EDAT- 1999/12/23 00:00
MHDA- 1999/12/23 00:01
CRDT- 1999/12/23 00:00
PHST- 1999/12/23 00:00 [pubmed]
PHST- 1999/12/23 00:01 [medline]
PHST- 1999/12/23 00:00 [entrez]
AID - 10.1074/jbc.274.53.37583 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 31;274(53):37583-90. doi: 10.1074/jbc.274.53.37583.