PMID- 10608757
OWN - NLM
STAT- MEDLINE
DCOM- 20000303
LR  - 20081121
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 181
IP  - 1
DP  - 2000 Jan
TI  - Transient T cell receptor beta-chain variable region-specific expansions of CD4+ 
      and CD8+ T cells during the early phase of pediatric human immunodeficiency virus
      infection: characterization of expanded cell populations by T cell receptor
      phenotyping.
PG  - 107-20
AB  - T cell receptor (TCR) repertoire perturbations are commonly detected in CD8+ T
      cells during adult primary human immunodeficiency virus (HIV) infection and have 
      been associated with HIV-specific cytotoxic T cell responses. By use of flow
      cytometry, transient high-level TCR beta-chain variable region-specific
      expansions of CD4+ and CD8+ T cells were observed more frequently in HIV-infected
      children than in children exposed to HIV who remained uninfected. TCR beta-chain 
      diversity analysis and diversity-specific polymerase chain reaction were used to 
      study the clonality of expanded CD4+ and CD8+ subsets. In CD8+ T cells,
      structural features of the complement-determining regions 3 were altered during
      the course of the expansion, and persistent TCR clonotypes were observed,
      consistent with antigen-driven selection. In contrast, TCR beta-chain variable
      region-specific expansions without clonotypic overrepresentation or persistence
      were observed in CD4+ T cells, possibly related to HIV-specific helper T cell
      responses or to the progressive destruction of the CD4+ cell compartment.
FAU - Soudeyns, H
AU  - Soudeyns H
AD  - Laboratoire d'Immunologie, Institut de Recherches Cliniques de Montreal, McGill
      University, Montreal, Canada. hsoudeyns@justine.umontreal.ca
FAU - Champagne, P
AU  - Champagne P
FAU - Holloway, C L
AU  - Holloway CL
FAU - Silvestri, G U
AU  - Silvestri GU
FAU - Ringuette, N
AU  - Ringuette N
FAU - Samson, J
AU  - Samson J
FAU - Lapointe, N
AU  - Lapointe N
FAU - Sekaly, R P
AU  - Sekaly RP
LA  - eng
GR  - AI-235516/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Child, Preschool
MH  - *Genes, T-Cell Receptor beta
MH  - HIV Infections/*immunology/transmission
MH  - HIV-1/*immunology
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Infectious Disease Transmission, Vertical
MH  - Longitudinal Studies
MH  - Lymphocyte Activation
MH  - Phenotype
MH  - Receptors, Antigen, T-Cell, alpha-beta/classification/*genetics
MH  - T-Lymphocyte Subsets/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 1999/12/23 09:00
MHDA- 2000/03/21 09:00
CRDT- 1999/12/23 09:00
PHST- 1999/12/23 09:00 [pubmed]
PHST- 2000/03/21 09:00 [medline]
PHST- 1999/12/23 09:00 [entrez]
AID - JID990455 [pii]
AID - 10.1086/315181 [doi]
PST - ppublish
SO  - J Infect Dis. 2000 Jan;181(1):107-20. doi: 10.1086/315181.