PMID- 10607915
OWN - NLM
STAT- MEDLINE
DCOM- 20000315
LR  - 20191103
IS  - 0945-053X (Print)
IS  - 0945-053X (Linking)
VI  - 18
IP  - 6
DP  - 1999 Dec
TI  - Tissue distribution of a novel cell binding protein, osteoadherin, in the rat.
PG  - 533-42
AB  - Osteoadherin is a cell binding keratan sulfate proteoglycan which was recently
      isolated from mineralized bovine bone and subsequently cloned and sequenced. For 
      studies of osteoadherin expression in rat tissues we isolated and sequenced a
      1.3-kbp partial cDNA covering most of the coding region using a rat calvaria cDNA
      library. The most 5' end of the cDNA was obtained by reverse transcription PCR
      from the bone total RNA preparation. The deduced, translated protein sequence
      containing 423 amino acid residues shows high sequence identity to mouse, bovine 
      and human osteoadherin except in the very acidic C-terminal region. However, the 
      rat counterpart showed a similarly high content of acidic amino acid residues.
      Ribonuclease protection assay showed osteoadherin mRNA to be expressed in femoral
      bone and calvaria tissues, while no expression was detected in cartilage, tendon 
      or liver. Using very sensitive nested RT-PCR, however, message was detected in
      femoral head, rib, tendon and bone marrow total RNA preparations. An antiserum
      specific for the rat C-terminal region of osteoadherin was generated and used for
      studies of protein distribution by immunohistochemistry during femoral head
      development. Osteoadherin was primarily present in bone trabeculae and no
      staining was seen in cartilage. In situ hybridization showed the strongest
      expression in osteoblasts close to the cartilage/bone interface of the growth
      plate and lower expression in diaphyseal osteoblasts. On maturation of the
      femoral head on day 60 some expression was detected immediately below the forming
      articular cartilage. Our data indicated that osteoadherin is primarily expressed 
      by osteoblasts and might have a role in regulation of mineralization.
FAU - Shen, Z
AU  - Shen Z
AD  - Section for Connective Tissue Biology, Department of Cell and Molecular Biology, 
      University of Lund, Lund, Sweden.
FAU - Gantcheva, S
AU  - Gantcheva S
FAU - Sommarin, Y
AU  - Sommarin Y
FAU - Heinegard, D
AU  - Heinegard D
LA  - eng
SI  - GENBANK/AF104362
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Matrix Biol
JT  - Matrix biology : journal of the International Society for Matrix Biology
JID - 9432592
RN  - 0 (Antibodies)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
RN  - 0 (osteoadherin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies
MH  - Base Sequence
MH  - Cattle
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Extracellular Matrix Proteins/genetics/immunology/*metabolism
MH  - Femur/embryology/metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Proteoglycans/genetics/immunology/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/12/23 09:00
MHDA- 2000/03/18 09:00
CRDT- 1999/12/23 09:00
PHST- 1999/12/23 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 1999/12/23 09:00 [entrez]
AID - S0945-053X(99)00048-7 [pii]
AID - 10.1016/s0945-053x(99)00048-7 [doi]
PST - ppublish
SO  - Matrix Biol. 1999 Dec;18(6):533-42. doi: 10.1016/s0945-053x(99)00048-7.