PMID- 10607840
OWN - NLM
STAT- MEDLINE
DCOM- 20000228
LR  - 20190513
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 9
IP  - 2
DP  - 2000 Jan 22
TI  - A novel human odorant-binding protein gene family resulting from genomic
      duplicons at 9q34: differential expression in the oral and genital spheres.
PG  - 289-301
AB  - Lipocalins are carrier proteins for hydrophobic molecules in many biological
      fluids. In the oral sphere (nasal mucus, saliva, tears), they have an
      environmental biosensor function and are involved in the detection of odours and 
      pheromones. Herein, we report the first identification of human lipocalins
      involved in odorant binding. They correspond to a gene family located on human
      chromosome 9q34 produced by genomic duplications: two new odorant-binding protein
      genes ( hOBP (IIa) and hOBP (IIb) ), the previously described tear lipocalin LCN1
      gene and two new LCN1 pseudogenes. Although 95% similar in sequence, the two hOBP
      (II) genes were differentially expressed in secretory structures. hOBP (IIa) was 
      strongly expressed in the nasal structures, salivary and lachrymal glands, and
      lung, therefore having an oral sphere profile. hOBP (IIb) was more strongly
      expressed in genital sphere organs such as the prostate and mammary glands. Both 
      were expressed in the male deferent ducts and placenta. Surprisingly,
      alternatively spliced mRNAs resulting in proteins with different C-termini were
      generated from each of the two genes. The single LCN1 gene in humans generated a 
      putative odorant-binding protein in nasal structures. Finally, based on the
      proposed successive genomic duplication history, we demonstrated the recruitment 
      of exons within intronic DNA generating diversity. This is consistent with a
      positive selection pressure in vertebrate evolution in the intron-late
      hypothesis.
FAU - Lacazette, E
AU  - Lacazette E
AD  - Laboratoire de Biochimie Medicale (Prof. B. Dastugue), INSERM U384, Faculte de
      Medecine, 28 place Henri Dunant, 63000 Clermont-Ferrand, France.
FAU - Gachon, A M
AU  - Gachon AM
FAU - Pitiot, G
AU  - Pitiot G
LA  - eng
SI  - GENBANK/AJ251020
SI  - GENBANK/AJ251021
SI  - GENBANK/AJ251022
SI  - GENBANK/AJ251023
SI  - GENBANK/AJ251024
SI  - GENBANK/AJ251025
SI  - GENBANK/AJ251026
SI  - GENBANK/AJ251027
SI  - GENBANK/AJ251028
SI  - GENBANK/AJ251029
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Carrier Proteins)
RN  - 0 (LCN1 protein, human)
RN  - 0 (Lipocalin 1)
RN  - 0 (Lipocalins)
RN  - 0 (OBP2A protein, human)
RN  - 0 (Receptors, Odorant)
RN  - 0 (odorant-binding protein)
SB  - IM
MH  - Alternative Splicing/genetics
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Carrier Proteins/biosynthesis/chemistry/*genetics/metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Female
MH  - *Gene Duplication
MH  - Gene Expression Regulation/genetics
MH  - Genitalia/*metabolism
MH  - Humans
MH  - Lipocalin 1
MH  - Lipocalins
MH  - Male
MH  - Molecular Sequence Data
MH  - Mouth/*metabolism
MH  - Multigene Family/*genetics
MH  - Organ Specificity
MH  - Receptors, Odorant/biosynthesis/*genetics/isolation & purification
MH  - Sequence Homology, Nucleic Acid
EDAT- 1999/12/23 09:00
MHDA- 2000/03/04 09:00
CRDT- 1999/12/23 09:00
PHST- 1999/12/23 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 1999/12/23 09:00 [entrez]
AID - ddd034 [pii]
AID - 10.1093/hmg/9.2.289 [doi]
PST - ppublish
SO  - Hum Mol Genet. 2000 Jan 22;9(2):289-301. doi: 10.1093/hmg/9.2.289.