PMID- 10607756
OWN - NLM
STAT- MEDLINE
DCOM- 20000224
LR  - 20190513
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 12
IP  - 1
DP  - 2000 Jan
TI  - Cellular responses to bacterial cell wall components are mediated through
      MyD88-dependent signaling cascades.
PG  - 113-7
AB  - MyD88 is an adaptor molecule essential for signaling via the Toll-like receptor
      (TLR)/IL-1 receptor family. TLR4 is a member of the TLR family and a point
      mutation in the Tlr4 gene causes hyporesponsiveness to lipopolysaccharide (LPS)
      in C3H/HeJ mice. We have previously shown that both TLR4- and MyD88-deficient
      mice are hyporesponsive to LPS. In this study we examined the responsiveness of
      these two knockout mice to various bacterial cell wall components. Cells from
      TLR4-deficient mice responded to several kinds of LPS, peptidoglycan and crude
      cell wall preparation from Gram-positive bacteria and mycobacterial lysates. In
      contrast, macrophages and splenocytes from MyD88-deficient mice did not respond
      to any of the bacterial components we tested. These results show that MyD88 is
      essential for the cellular response to bacterial cell wall components.
FAU - Takeuchi, O
AU  - Takeuchi O
AD  - Department of Host Defense, Research Institute for Microbial Diseases, Osaka
      University and CREST of Japan Science and Technology Corp., 3-1 Yamada-oka,
      Suita, Osaka 565-0871, Japan.
FAU - Takeda, K
AU  - Takeda K
FAU - Hoshino, K
AU  - Hoshino K
FAU - Adachi, O
AU  - Adachi O
FAU - Ogawa, T
AU  - Ogawa T
FAU - Akira, S
AU  - Akira S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Peptidoglycan)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Antigens, Differentiation/genetics/*metabolism
MH  - Cell Wall/*immunology
MH  - *Drosophila Proteins
MH  - Gram-Negative Bacteria/immunology
MH  - Gram-Positive Bacteria/immunology
MH  - Lipopolysaccharides/*immunology
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Knockout
MH  - Mycobacterium/immunology
MH  - Myeloid Differentiation Factor 88
MH  - Peptidoglycan/*immunology
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - *Receptors, Immunologic
MH  - Signal Transduction
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
EDAT- 1999/12/23 09:00
MHDA- 2000/02/26 09:00
CRDT- 1999/12/23 09:00
PHST- 1999/12/23 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 1999/12/23 09:00 [entrez]
AID - 10.1093/intimm/12.1.113 [doi]
PST - ppublish
SO  - Int Immunol. 2000 Jan;12(1):113-7. doi: 10.1093/intimm/12.1.113.