PMID- 10607567
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190728
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 23
DP  - 1999 Dec 2
TI  - Identification of the Nef-associated kinase as p21-activated kinase 2.
PG  - 1407-10
AB  - The Nef protein of primate immunodeficiency viruses plays an important role in
      the pathogenesis of acquired immunodeficiency syndrome (AIDS) [1] [2]. The
      interaction of Nef with the Nef-associated kinase (NAK) is one of the most
      conserved properties of different human and simian immunodeficiency virus (HIV
      and SIV) Nef alleles. The role of NAK association is currently not known but it
      has been implicated in enhanced viral infectivity in cell culture and in disease 
      progression in SIV-infected macaques [3]. Previous studies have indicated that
      NAK shares many features with the p21-activated kinases (PAKs) [3], but the
      molecular identity of NAK has remained unknown. We have generated specific
      antisera against PAKs 1-3, and expressed these kinases individually as
      epitope-tagged proteins. By using these reagents in experiments involving partial
      proteolytic mapping, and exploiting the unique ability of PAK2 to serve as a
      caspase substrate, we have positively identified NAK as PAK2. Interestingly,
      although ectopic PAK2 overexpression efficiently replaced endogenous PAK2 from
      the complex with Nef, the total Nef-associated PAK2 activity was not increased,
      indicating the abundance of another cellular factor(s) as the limiting factor in 
      Nef-PAK2 complex formation. Identification of NAK as PAK2 should now facilitate
      elucidation of its role as a mediator of the pathogenic effects of Nef.
FAU - Renkema, G H
AU  - Renkema GH
AD  - Institute of Medical Technology, University of Tampere, Tampere, Finland.
FAU - Manninen, A
AU  - Manninen A
FAU - Mann, D A
AU  - Mann DA
FAU - Harris, M
AU  - Harris M
FAU - Saksela, K
AU  - Saksela K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (Immune Sera)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.1 (PAK1 protein, human)
RN  - EC 2.7.11.1 (PAK2 protein, human)
RN  - EC 2.7.11.1 (PAK3 protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
SB  - IM
SB  - X
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Autoradiography
MH  - Cell Line
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epitopes/immunology
MH  - Humans
MH  - Immune Sera/blood
MH  - Precipitin Tests
MH  - Protein-Serine-Threonine Kinases/genetics/immunology/*isolation &
      purification/metabolism
MH  - Recombinant Proteins/biosynthesis
MH  - Transfection
MH  - p21-Activated Kinases
EDAT- 1999/12/23 00:00
MHDA- 1999/12/23 00:01
CRDT- 1999/12/23 00:00
PHST- 1999/12/23 00:00 [pubmed]
PHST- 1999/12/23 00:01 [medline]
PHST- 1999/12/23 00:00 [entrez]
AID - S0960-9822(00)80086-X [pii]
AID - 10.1016/s0960-9822(00)80086-x [doi]
PST - ppublish
SO  - Curr Biol. 1999 Dec 2;9(23):1407-10. doi: 10.1016/s0960-9822(00)80086-x.