PMID- 10607561
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20190728
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 23
DP  - 1999 Dec 2
TI  - Developmental and cell biological functions of the Drosophila DEAD-box protein
      abstrakt.
PG  - 1373-81
AB  - BACKGROUND: DEAD-box proteins are a large family of proteins found in bacteria,
      plants and animals, but only few have been analysed functionally. They are
      involved in the regulation of various aspects of RNA processing and metabolism,
      including splicing, transport and translation. The study of their function in
      multicellular organisms has been restricted to a few special cases, such as the
      Vasa protein in the fruit fly Drosophila. RESULTS: We show that abstrakt, a gene 
      originally identified genetically by its effect on axon outgrowth and
      fasciculation of the Bolwig nerve, encodes a new Drosophila DEAD-box protein of
      which the closest homologue is a human gene of unknown function. Using
      temperature-sensitive alleles to assay its function, we found that abstrakt is
      essential for survival at all stages throughout the life cycle of the fly.
      Mutants show specific defects in many developmental processes, including
      cell-shape changes, localisation of RNA and apoptosis. CONCLUSIONS: Abstrakt is
      not globally required for RNA splicing, transport, subcellular localisation or
      translation. Nevertheless, there is a widespread requirement for Abstrakt during 
      post-transcriptional gene expression. Abstrakt must affect processing of specific
      subsets of RNAs, suggesting that differential post-translational control during
      development is more common than previously suspected.
FAU - Irion, U
AU  - Irion U
AD  - Institute of Genetics, University of Cologne, Wellcome/CRC Institute, Cologne,
      Cambridge, D-50931, CB2 1QR, Germany, UK.
FAU - Leptin, M
AU  - Leptin M
LA  - eng
SI  - GENBANK/AF187729
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Abs protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/genetics
MH  - Base Sequence
MH  - *Body Patterning
MH  - Cell Nucleus/metabolism
MH  - *Cell Physiological Phenomena/drug effects
MH  - Cell Polarity/genetics
MH  - Cytoplasm/metabolism
MH  - Drosophila/*chemistry/*embryology/growth & development
MH  - *Drosophila Proteins
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - In Situ Hybridization
MH  - In Situ Nick-End Labeling
MH  - Insect Proteins/genetics/metabolism/pharmacology/*physiology
MH  - Life Cycle Stages/genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - *Nuclear Proteins
MH  - RNA/analysis/drug effects/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/12/23 00:00
MHDA- 1999/12/23 00:01
CRDT- 1999/12/23 00:00
PHST- 1999/12/23 00:00 [pubmed]
PHST- 1999/12/23 00:01 [medline]
PHST- 1999/12/23 00:00 [entrez]
AID - S0960-9822(00)80082-2 [pii]
AID - 10.1016/s0960-9822(00)80082-2 [doi]
PST - ppublish
SO  - Curr Biol. 1999 Dec 2;9(23):1373-81. doi: 10.1016/s0960-9822(00)80082-2.